Anti-MCM7/PRL抗体[47DC141] (ab2360)
Key features and details
- Mouse monoclonal [47DC141] to MCM7/PRL
- Suitable for: IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Dog, Human, Xenopus laevis
- Isotype: IgG1
概述
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产品名称
Anti-MCM7/PRL抗体[47DC141]
参阅全部 MCM7/PRL 一抗 -
描述
小鼠单克隆抗体[47DC141] to MCM7/PRL -
宿主
Mouse -
经测试应用
适用于: IHC-P, Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Dog, Human, Xenopus laevis -
免疫原
Recombinant full length protein corresponding to Human MCM7/PRL.
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阳性对照
- Breast carcinoma, MAD109 cell lysate, PC12 cell lysate.
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常规说明
This product was previously labelled as MCM7
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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纯度
IgG fraction -
克隆
单克隆 -
克隆编号
47DC141 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab2360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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IHC-P | 1/50 - 1/100. This is when using an ABC method for 30 minutes at room temperature. Sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. | |
Flow Cyt | 1/20. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ICC/IF | Use a concentration of 1 µg/ml. |
靶标
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功能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. -
序列相似性
Belongs to the MCM family.
Contains 1 MCM domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4176 Human
- Entrez Gene: 17220 Mouse
- Entrez Gene: 288532 Rat
- Omim: 600592 Human
- SwissProt: P33993 Human
- SwissProt: Q61881 Mouse
- Unigene: 438720 Human
- Unigene: 378965 Mouse
see all -
别名
- CDABP0042 antibody
- CDC 47 antibody
- CDC47 antibody
see all
图片
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This image shows immunostaining of MCM7/PRL in rat brain endothelial cells. Brain endothelial cells were co-cultured with neuronal precursor cells and the nuclear staining represents cells in cell cycle. Primary antibody MCM7/PRL (ab2360) was used at 1:50 dilution, incubated overnight at 4 oC. Secondary antibody - Alexafluor (488 nm) at 1:200 dilution, incubated for 2 hours at room temperature.
The picture was kindly supplied by Dr Joseph Corteza Lim and Dr Margery Barrand from University of Cambridge, Department of Pharmacology.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody [47DC141] (ab2360)Image kindly supplied by Dr Karin Birkenkamp-Demtroeder through Abreview
ab2360 staining MCM7/PRL in human bladder cancer tissue sections by Immunohistochemistry (formalin fixed sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Tissue was blocked with 5% BSA for 1 hour at room temperature followed by incubation with the primary antibody at a 1/1200 dilution for 1 hour. A HRP-conjugated goat anti-mouse polyclonal was used as secondary antibody un-diluted.
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ICC/IF image of MCM7/PRL (ab2360) stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2360, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Overlay histogram showing HeLA cells stained with MCM7/PRL (ab2360) (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2360, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody [47DC141] (ab2360)
ab2360 staining MCM7/PRL in formalin-fixed, paraffin-embedded Human breast carcinoma tissue tissue by Immunohistochemistry.
实验方案
数据表及文件
文献 (23)
ab2360 被引用在 23 文献中.
- Kovary KM et al. Expression variation and covariation impair analog and enable binary signaling control. Mol Syst Biol 14:e7997 (2018). PubMed: 29759982
- Drissi R et al. Destabilization of the MiniChromosome Maintenance (MCM) complex modulates the cellular response to DNA double strand breaks. Cell Cycle 17:2593-2609 (2018). PubMed: 30516086
- Charrasse S et al. Ensa controls S-phase length by modulating Treslin levels. Nat Commun 8:206 (2017). PubMed: 28785014
- Bai G et al. Chronic DNA Replication Stress Reduces Replicative Lifespan of Cells by TRP53-Dependent, microRNA-Assisted MCM2-7 Downregulation. PLoS Genet 12:e1005787 (2016). WB ; Mouse . PubMed: 26765334
- Guan B et al. Minichromosome maintenance complex component 7 has an important role in the invasion of papillary urothelial neoplasia. Oncol Lett 10:946-950 (2015). PubMed: 26622601