Anti-MCM6抗体(ab4458)

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Key features and details

  • Rabbit polyclonal to MCM6
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-MCM6抗体
    参阅全部 MCM6 一抗

  • 描述

    兔多克隆抗体to MCM6

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: IP

  • 种属反应性

    与反应: Human
    预测可用于: Rat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan

  • 免疫原

    Synthetic peptide (Human) - 31 amino acids which represented a portion of human MCM 6 encoded by exon 17.

性能

应用

Our Abpromise guarantee covers the use of ab4458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 91 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明
    Is unsuitable for IP.

    • 靶标

    • 功能

      Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

    • 序列相似性

      Belongs to the MCM family.
      Contains 1 MCM domain.

    • 翻译后修饰

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • 细胞定位

      Nucleus. Binds to chromatin during G1 and detach from it during S phase.
    • Information by UniProt

    • 数据库链接

      see all

    • 别名

      • DNA replication licensing factor MCM6 antibody
      • MCG40308 antibody
      • Mcm6 antibody
      • MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) antibody
      • MCM6 minichromosome maintenance deficient 6 antibody
      • MCM6_HUMAN antibody
      • Minichromosome maintenance complex component 6 antibody
      • Minichromosome maintenance deficient (mis5 S. pombe) 6 antibody
      • Minichromosome maintenance deficient 6 homolog antibody
      • Minichromosome maintenance protein 6 antibody
      • Mis5 antibody
      • MIS5 homolog antibody
      • p105MCM antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (ab4458)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (ab4458)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling MCM6 with ab4458 at 1/1000 (1µg/ml). Detection: DAB.
    • Western blot - Anti-MCM6 antibody (ab4458)
      Western blot - Anti-MCM6 antibody (ab4458)

      Sample: HeLa cell RIPA extract (50 µg).

      Antibody: ab4458 at 0.2 µg/ml incubated for 1 hour.

      Sample: HeLa cell RIPA extract (50 µg).

      Antibody: ab4458 at 0.2 µg/ml incubated for 1 hour.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (ab4458)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (ab4458)
      IHC image of ab4458 staining in normal human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4458, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    文献 (5)

    发表研究结果有使用 ab4458？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab4458 被引用在 5 文献中.

    • Chen QY  et al. CDK5RAP3 Inhibits the Translocation of MCM6 to Influence the Prognosis in Gastric Cancer. J Cancer 10:4488-4498 (2019). PubMed: 31528213
    • Quimbaya M  et al. Deregulation of the Replisome Factor MCMBP Prompts Oncogenesis in Colorectal Carcinomas through Chromosomal Instability. Neoplasia 16:694-709 (2014). Human . PubMed: 25246271
    • Gronnier C  et al. The MUC1 mucin regulates the tumorigenic properties of human esophageal adenocarcinomatous cells. Biochim Biophys Acta 1843:2432-7 (2014). PubMed: 25003315
    • Kawabata T  et al. A reduction of licensed origins reveals strain-specific replication dynamics in mice. Mamm Genome 22:506-17 (2011). WB ; Mouse . PubMed: 21611832
    • Takahashi N  et al. The MCM-binding protein ETG1 aids sister chromatid cohesion required for postreplicative homologous recombination repair. PLoS Genet 6:e1000817 (2010). WB ; Human . PubMed: 20090939

    客户评价及客户问答

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-MCM6 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human cerebral organoid)
    Specification
    Human cerebral organoid
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Radhika Menon

    Verified customer

    提交于 Dec 13 2017

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