Anti-MCM6抗体(ab3730)

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Western blot - Anti-MCM6 antibody (ab3730)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (ab3730)

Key features and details

  • Goat polyclonal to MCM6
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-MCM6抗体
    参阅全部 MCM6 一抗

  • 描述

    山羊多克隆抗体to MCM6

  • 宿主

    Goat

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide (Human) - which represented a portion of human Minichromosome Maintenance Deficient 6 encoded by exon 17.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
IHC-P
Human
WB
Human
All applications
Mouse
Horse
Guinea pig
Cow
Dog
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
应用 Ab评论 说明
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/2000 - 1/10000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
说明
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/2000 - 1/10000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).

靶标

  • 功能

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

  • 序列相似性

    Belongs to the MCM family.
    Contains 1 MCM domain.

  • 翻译后修饰

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 细胞定位

    Nucleus. Binds to chromatin during G1 and detach from it during S phase.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • DNA replication licensing factor MCM6 antibody
    • MCG40308 antibody
    • Mcm6 antibody
    • MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) antibody
    • MCM6 minichromosome maintenance deficient 6 antibody
    • MCM6_HUMAN antibody
    • Minichromosome maintenance complex component 6 antibody
    • Minichromosome maintenance deficient (mis5 S. pombe) 6 antibody
    • Minichromosome maintenance deficient 6 homolog antibody
    • Minichromosome maintenance protein 6 antibody
    • Mis5 antibody
    • MIS5 homolog antibody
    • p105MCM antibody
    see all

图片

  • Western blot - Anti-MCM6 antibody (ab3730)
    Western blot - Anti-MCM6 antibody (ab3730)

    Sample: Hela cell RIPA extract (60µg).

    Antibody: Affinity purified Goat anti-MCM 6 (ab3730) diluted 1 / 4000.

    Detection: ECL.

     

    Sample: Hela cell RIPA extract (60µg).

    Antibody: Affinity purified Goat anti-MCM 6 (ab3730) diluted 1 / 4000.

    Detection: ECL.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (ab3730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (ab3730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MCM6 with ab3730 at 1/1000 (1µg/ml). Detection: DAB.

文献 (1)

发表研究结果有使用 ab3730？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab3730 被引用在 1 文献中.

  • Kim TM  et al. Gene expression analysis reveals the cell cycle and kinetochore genes participating in ischemia reperfusion injury and early development in kidney. PLoS One 6:e25679 (2011). IHC-Fr ; Rat . PubMed: 21980527

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