Key features and details
- Rabbit polyclonal to MCM4
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human, Xenopus laevis
- Isotype: IgG
参阅全部 MCM4 一抗
经测试应用适用于: ICC/IF, IHC-P, WBmore details
种属反应性与反应: Mouse, Human, Xenopus laevis
预测可用于: Rabbit, Horse, Guinea pig, Cow, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
Synthetic peptide (Human) - 32 amino acids which represented a portion of human MCM 4 encoded within exon 14.
- IHC-P: FFPE human testis tissue sections.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab4459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 96.6 kDa).|
功能Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
序列相似性Belongs to the MCM family.
Contains 1 MCM domain.
- Information by UniProt
- 19G antibody
- AI325074 antibody
- AU045576 antibody
HeLa cell RIPA extract incubated with 0.2
µg/ml of ab4459 for 1 hour. HeLa cell RIPA extract incubated with 0.2 µg/ml of ab4459 for 1 hour.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and mouse squamous cell carcinoma (right) tissues labelling MCM4 with ab4459 at 1/1000 (1µg/ml). Detection: DAB.
IHC image of MCM4 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4459, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-MCM4 antibody (ab4459) at 1/2000 dilution + HeLa nuclear cell lysate. at 20 µg
Goat anti rabbit HRP. at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 96.6 kDa
This image is an edited version of an image received courtesy of an Abreview submitted by Jason DeHart on 22 August 2005. We do not have any further information relating to this image.
ICC/IF image of ab4459 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4459, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab4459 被引用在 21 文献中.
- Prikrylova T et al. 5-hydroxymethylcytosine Marks Mammalian Origins Acting as a Barrier to Replication. Sci Rep 9:11065 (2019). PubMed: 31363131
- Starokadomskyy P et al. NK cell defects in X-linked pigmentary reticulate disorder. JCI Insight 4:N/A (2019). PubMed: 31672938
- Cortone G et al. Interaction of the Warsaw breakage syndrome DNA helicase DDX11 with the replication fork-protection factor Timeless promotes sister chromatid cohesion. PLoS Genet 14:e1007622 (2018). PubMed: 30303954
- Charrasse S et al. Ensa controls S-phase length by modulating Treslin levels. Nat Commun 8:206 (2017). PubMed: 28785014
- Hiraga SI et al. Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation. EMBO Rep 18:403-419 (2017). WB ; Human . PubMed: 28077461