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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis Other

Anti-MCM2抗体(ab3723)

  • Datasheet
  • SDS
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Western blot - Anti-MCM2 antibody (ab3723)
  • Western blot - Anti-MCM2 antibody (ab3723)

Key features and details

  • Goat polyclonal to MCM2
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-MCM2抗体
    参阅全部 MCM2 一抗
  • 描述

    山羊多克隆抗体to MCM2
  • 宿主

    Goat
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human MCM2 aa 1-50.
    Database link: NP_0045117.2

  • 阳性对照

    • HeLa cell lysate.
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7.00
    Preservative: 0.09% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

应用

Our Abpromise guarantee covers the use of ab3723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).

靶标

  • 功能

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
  • 序列相似性

    Belongs to the MCM family.
    Contains 1 MCM domain.
  • 翻译后修饰

    Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
  • 细胞定位

    Nucleus.
  • Target information above from: UniProt accession P49736 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4171 Human
    • Entrez Gene: 17216 Mouse
    • Omim: 116945 Human
    • SwissProt: P49736 Human
    • SwissProt: P97310 Mouse
    • Unigene: 477481 Human
    • Unigene: 16711 Mouse
    • 别名

      • BM28 antibody
      • CCNL 1 antibody
      • CCNL1 antibody
      • CDC like 1 antibody
      • cdc19 antibody
      • CDCL 1 antibody
      • CDCL1 antibody
      • Cell devision cycle like 1 antibody
      • Cyclin like 1 antibody
      • D3S3194 antibody
      • DNA replication licensing factor MCM2 antibody
      • KIAA0030 antibody
      • MCM 2 antibody
      • MCM2 antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin antibody
      • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
      • MCM2 minichromosome maintenance deficient 2, mitotin antibody
      • MCM2_HUMAN antibody
      • MGC10606 antibody
      • Minichromosome maintenance complex component 2 antibody
      • Minichromosome maintenance deficient 2 (mitotin) antibody
      • Minichromosome maintenance deficient 2 mitotin antibody
      • Minichromosome maintenance protein 2 antibody
      • Minichromosome maintenance protein 2 homolog antibody
      • Mitotin antibody
      • Nuclear protein BM28 antibody
      • OTTHUMP00000216047 antibody
      • OTTHUMP00000216050 antibody
      see all

    图片

    • Western blot - Anti-MCM2 antibody (ab3723)
      Western blot - Anti-MCM2 antibody (ab3723)

      Sample: Hela cell RIPA extract (60µg).

      Antibody: Affinity purified Goat anti-MCM 2 (ab3723) diluted 1 / 4000.

      Detection: ECL.

      Sample: Hela cell RIPA extract (60µg). Antibody: Affinity purified Goat anti-MCM 2 (ab3723) diluted 1 / 4000. Detection: ECL.
    • Western blot - Anti-MCM2 antibody (ab3723)
      Western blot - Anti-MCM2 antibody (ab3723)
      All lanes : Anti-MCM2 antibody (ab3723) at 0.5 µg/ml

      Lane 1 : NIH3T3 cells
      Lane 2 : TCMK-1 cells
      Lane 3 : 4T1 cells
      Lane 4 : CT26.WT cells

      Lysates/proteins at 50 µg per lane.

      Predicted band size: 120 kDa



      Detection:Chemiluminescence with an exposed time of 3 minutes.

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (0)

    发表研究结果有使用 ab3723?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3723 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Immunohistochemistry (Frozen sections) abreview for Anti-MCM2 antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Mouse Embryo (day 8.5))
    Specification
    Mouse Embryo (day 8.5)
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Saponin
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
    Read More

    Derek Passer

    Verified customer

    提交于 Aug 26 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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