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Cell Biology Apoptosis Intracellular Bcl2 Family
使用敲除细胞株进行验证重组RabMAb

重组Anti-MCL1抗体[Y37] (ab32087)

  • Datasheet
  • SDS
Reviews (9)Q&A (6)References (87)

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Western blot - Anti-MCL1 antibody [Y37] (ab32087)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCL1 antibody [Y37] (ab32087)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody [Y37] (ab32087)
  • Flow Cytometry - Anti-MCL1 antibody [Y37] (ab32087)
  • Western blot - Anti-MCL1 antibody [Y37] (ab32087)
  • Western blot - Anti-MCL1 antibody [Y37] (ab32087)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody [Y37] (ab32087)
  • Flow Cytometry - Anti-MCL1 antibody [Y37] (ab32087)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody [Y37] (ab32087)
  • Anti-MCL1 antibody [Y37] (ab32087)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y37] to MCL1
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

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概述

  • 产品名称

    Anti-MCL1抗体[Y37]
    参阅全部 MCL1 一抗
  • 描述

    兔单克隆抗体[Y37] to MCL1
  • 宿主

    Rabbit
  • 特异性

    ab32087 recognises MCL1. The antibody does not cross-react with other Bcl-2 family members.
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide within Human MCL1 aa 100-200. The exact sequence is proprietary.
    Database link: Q07820
    (Peptide available as ab199979)

  • 阳性对照

    • WB: Human lung, lung cancer and liver lysates; HEK293T, A431, Ramos, H:-60, HeLa, MCF7 and HepG2 whole cell lysate (ab7900). IHC-P: Human colon adenocarcinoma tissue. ICC/IF: HCT 116, MCF7 and H1299 cells. Flow Cyt: Ramos and A431 cells.
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    Y37
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

相关产品

  • Alternative Versions

    • Anti-MCL1 antibody [Y37] - BSA and Azide free (ab186822)
    • Alexa Fluor® 647 Anti-MCL1 antibody [Y37] (ab197035)
    • Alexa Fluor® 488 Anti-MCL1 antibody [Y37] (ab197529)
    • PE Anti-MCL1 antibody [Y37] (ab209289)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human MCL1 knockout HEK-293T cell line (ab266838)
  • KO cell lysates

    • Human MCL1 knockout HEK-293T cell lysate (ab256986)
  • KO cell pellets

    • Human MCL1 knockout HEK293T cell pellet (ab278986)
  • Positive Controls

    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human MCL1 protein (ab131682)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Mouse
Rat
应用 Ab评论 说明
ICC/IF (2)
1/100 - 1/500.
WB (6)
1/1000 - 1/5000. Predicted molecular weight: 37 kDa.

 

IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt
1/250.

For unpurified use 1 ug for 106 cells. (For lot-specific stock concentration, please contact Abcam).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

说明
ICC/IF
1/100 - 1/500.
WB
1/1000 - 1/5000. Predicted molecular weight: 37 kDa.

 

IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt
1/250.

For unpurified use 1 ug for 106 cells. (For lot-specific stock concentration, please contact Abcam).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
  • 序列相似性

    Belongs to the Bcl-2 family.
  • 翻译后修饰

    Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
    Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
    Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
  • 细胞定位

    Membrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria.
  • Target information above from: UniProt accession Q07820 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4170 Human
    • Entrez Gene: 17210 Mouse
    • Entrez Gene: 60430 Rat
    • Omim: 159552 Human
    • SwissProt: Q07820 Human
    • SwissProt: P97287 Mouse
    • SwissProt: Q9Z1P3 Rat
    • Unigene: 632486 Human
    • Unigene: 1639 Mouse
    • Unigene: 161889 Rat
    see all
  • 别名

    • Bcl 2 related protein EAT/mcl1 antibody
    • Bcl-2-like protein 3 antibody
    • Bcl-2-related protein EAT/mcl1 antibody
    • BCL2 related antibody
    • Bcl2-L-3 antibody
    • BCL2L3 antibody
    • EAT antibody
    • Induced myeloid leukemia cell differentiation protein Mcl 1 antibody
    • Induced myeloid leukemia cell differentiation protein Mcl-1 antibody
    • MCL 1 antibody
    • MCL1 antibody
    • MCL1-ES antibody
    • mcl1/EAT antibody
    • MCL1_HUMAN antibody
    • MCL1L antibody
    • MCL1S antibody
    • MGC104264 antibody
    • MGC1839 antibody
    • Myeloid Cell Leukemia 1 antibody
    • Myeloid cell leukemia ES antibody
    • Myeloid cell leukemia sequence 1 antibody
    • Myeloid cell leukemia sequence 1 BCL2 related antibody
    • Myeloid cell leukemia sequence 1 isoform 1 antibody
    • OTTHUMP00000032794 antibody
    • OTTHUMP00000032795 antibody
    • TM antibody
    see all

图片

  • Western blot - Anti-MCL1 antibody [Y37] (ab32087)
    Western blot - Anti-MCL1 antibody [Y37] (ab32087)
    All lanes : Anti-MCL1 antibody [Y37] (ab32087) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : MCL1 knockout HEK293T cell lysate
    Lane 3 : Ramos cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab32087 observed at 37 kDa. Red - loading control ab7291 observed at 50 kDa. 

     ab32087  Anti-MCL1 antibody [Y37]  was shown to specifically react with MCL1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266838 (knockout cell lysate ab256986) was used.  Wild-type and MCL1 knockout samples were subjected to SDS-PAGE.  ab32087 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCL1 antibody [Y37] (ab32087)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCL1 antibody [Y37] (ab32087)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon adenocarcinoma tissue labelling MCL1 with purified ab32087 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
  • Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody [Y37] (ab32087)
    Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody [Y37] (ab32087)

    Immunocytochemistry/Immunofluorescence analysis of HCT 116 (human colorectal carcinoma cell line) cells labelling MCL1 with purified ab32087 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Flow Cytometry - Anti-MCL1 antibody [Y37] (ab32087)
    Flow Cytometry - Anti-MCL1 antibody [Y37] (ab32087)

    Flow Cytometry analysis of Ramos (human Burkitt's lymphoma cell line) cells labelling MCL1 with purified ab32087 at 1/250 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Western blot - Anti-MCL1 antibody [Y37] (ab32087)
    Western blot - Anti-MCL1 antibody [Y37] (ab32087)
    All lanes : Anti-MCL1 antibody [Y37] (ab32087) at 1/1000 dilution

    Lane 1 : Human lung tissue with NFDM/TBST
    Lane 2 : Human lung cancer tissue with NFDM/TBST
    Lane 3 : Human liver tissue with NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated. at 1/1000 dilution

    Predicted band size: 37 kDa



    Exposure time for samples 1-3: 15 seconds; exposure time for samples 4-6: 1 minute.

    Additional bands: We are unsure as to the identity of these extra bands.

  • Western blot - Anti-MCL1 antibody [Y37] (ab32087)
    Western blot - Anti-MCL1 antibody [Y37] (ab32087)
    All lanes : Anti-MCL1 antibody [Y37] (ab32087) at 1/1000 dilution

    Lane 1 : Ramos (human Burkitt's lymphoma cell line) cell lysate with NFDM/TBST
    Lane 2 : HL-60 (human promyelocytic leukemia cell line) cell lysate with NFDM/TBST
    Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate with NFDM/TBST
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate with NFDM/TBST
    Lane 5 : MCF7 (human breast adenocarcinoma cell line) cell lysate with NFDM/TBST
    Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate with NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG (H+L), peroxidase conjugated. at 1/1000 dilution

    Predicted band size: 37 kDa


    Exposure time: 15 seconds


    Additional bands: We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody [Y37] (ab32087)
    Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody [Y37] (ab32087)This image is courtesy of an anonymous Abreview.

    Immunocytochemistry/Immunofluorescence analysis of H1299 cells labelling MCL1 with unpurified ab32087. Cells were PFA-fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 3% Serum for 1 hour at 24°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 24°C. The secondary antibody was an Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/2000. DAPI (blue) was used as the nuclear counterstain.

    See Abreview

  • Flow Cytometry - Anti-MCL1 antibody [Y37] (ab32087)
    Flow Cytometry - Anti-MCL1 antibody [Y37] (ab32087)

    Flow Cytometry analysis of A431 (human epidermoid carcinoma cell line) cells labelling MCL1 with unpurified ab32087 (red line). Cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32087, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. 

    Black - Isotype control, rabbit monoclonal IgG.

    Acquisition of >5,000 events was performed. This antibody gave a decreased signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody [Y37] (ab32087)
    Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody [Y37] (ab32087)

    Immunocytochemistry/Immunofluorescence analysis of HCT 116 (human colorectal carcinoma cell line) cells treated with wogonin (ab142471) labelling MCL1 with unpurified ab32087. Decrease of MCL1 expression correlates with increased concentration of wogonin, as described in literature. Cells were incubated at 37°C for 2h in media containing different concentrations of ab142471 (wogonin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32087 (1/100) dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

  • Anti-MCL1 antibody [Y37] (ab32087)
    Anti-MCL1 antibody [Y37] (ab32087)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (87)

发表研究结果有使用 ab32087?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab32087 被引用在 87 文献中.

  • Knoll G  et al. NOXA-dependent contextual synthetic lethality of BCL-XL inhibition and "osmotic reprogramming" in colorectal cancer. Cell Death Dis 11:257 (2020). PubMed: 32312973
  • Yuan B  et al. Role of Bcl-2 on drug resistance in breast cancer polyploidy-induced spindle poisons. Oncol Lett 19:1701-1710 (2020). PubMed: 32194662
  • Xia P  et al. MicroRNA-377 exerts a potent suppressive role in osteosarcoma through the involvement of the histone acetyltransferase 1-mediated Wnt axis. J Cell Physiol 234:22787-22798 (2019). PubMed: 31152456
  • Du Q  et al. Propofol inhibits proliferation and epithelial-mesenchymal transition of MCF-7 cells by suppressing miR-21 expression. Artif Cells Nanomed Biotechnol 47:1265-1271 (2019). PubMed: 30942630
  • Liu B  et al. LncRNA ANRIL protects against oxygen and glucose deprivation (OGD)-induced injury in PC-12 cells: potential role in ischaemic stroke. Artif Cells Nanomed Biotechnol 47:1384-1395 (2019). PubMed: 31174432
View all Publications for this product

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1-9 of 9 Abreviews

Immunocytochemistry/ Immunofluorescence abreview for Anti-MCL1 antibody [Y37]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (GBM primary culture)
Permeabilization
Yes - PBST 0.3%
Specification
GBM primary culture
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

Dr. Matthias Dedobbeleer

Verified customer

提交于 Sep 12 2018

Western blot abreview for Anti-MCL1 antibody [Y37]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U87MG cells)
Gel Running Conditions
Non-reduced Denaturing (12)
Loading amount
50 µg
Treatment
10Gray Irradiation
Specification
U87MG cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Dr. Matthias Dedobbeleer

Verified customer

提交于 Jul 18 2018

Western blot abreview for Anti-MCL1 antibody [Y37]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma primary cells)
Loading amount
30 µg
Specification
Glioblastoma primary cells
Gel Running Conditions
Reduced Denaturing (gradient gel 5-15%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Read More

Abcam user community

Verified customer

提交于 Nov 21 2012

Western blot abreview for Anti-MCL1 antibody [Y37]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblast)
Loading amount
20 µg
Specification
Mouse Embryonic Fibroblast
Gel Running Conditions
Reduced Denaturing (10% Bis Bris)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Dec 31 2010

Western blot abreview for Anti-MCL1 antibody [Y37]

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293T)
Loading amount
20 µg
Specification
HEK 293T
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Dec 30 2010

Immunoprecipitation abreview for Anti-MCL1 antibody [Y37]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - other (HELA)
Total protein in input
300 µg
Specification
HELA
Immuno-precipitation step
Protein A
Read More

Abcam user community

Verified customer

提交于 Nov 29 2010

Immunocytochemistry/ Immunofluorescence abreview for Anti-MCL1 antibody [Y37]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (H1299)
Specification
H1299
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-x
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Read More

Abcam user community

Verified customer

提交于 Nov 25 2010

Western blot abreview for Anti-MCL1 antibody [Y37]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (HELA)
Loading amount
30 µg
Specification
HELA
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Abcam user community

Verified customer

提交于 Nov 25 2010

Western blot abreview for Anti-MCL1 antibody [Y37]

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Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Primary keratinocytes)
Loading amount
30 µg
Specification
Primary keratinocytes
Treatment
15mJ/cm2 UVB
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Dr. Christine Tomlins

Verified customer

提交于 Oct 29 2009

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