Anti-MCL1抗体(ab28147)
Key features and details
- Rabbit polyclonal to MCL1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rabbit, Guinea pig, Dog, Human, Pig, Monkey
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-MCL1抗体
参阅全部 MCL1 一抗 -
描述
兔多克隆抗体to MCL1 -
宿主
Rabbit -
特异性
This antibody detects an ~42/43 kDa protein doublet, corresponding to the expected molecular mass of Mcl1 on SDS-PAGE immunoblots. The antibody specificity is confirmed by peptide inhibition studies. An additional protein at ~33kDa may also be detected. Other additional proteins may also be seen in some samples. -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Rabbit, Guinea pig, Dog, Human, Pig, Monkey -
免疫原
Synthetic peptide corresponding to Human MCL1 aa 121-139 conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:SPEEELDGYEPEPLGKRPA
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阳性对照
- ICC/IF: Human HeLa cells; IHC-P: Human breast cancer tissue; WB: Hela, MDCK and RK-13 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28147于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 42-43 kDa.
0.1 µg/ml was sufficient for detection of Mcl1 in 20µg of HeLa Heat Shocked Cell Lysate by ECL immunoblot analysis. |
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ICC/IF |
Use at an assay dependent concentration.
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说明 |
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IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 42-43 kDa. 0.1 µg/ml was sufficient for detection of Mcl1 in 20µg of HeLa Heat Shocked Cell Lysate by ECL immunoblot analysis. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis. -
序列相似性
Belongs to the Bcl-2 family. -
翻译后修饰
Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites. -
细胞定位
Membrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria. - Information by UniProt
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数据库链接
- Entrez Gene: 403537 Dog
- Entrez Gene: 4170 Human
- Entrez Gene: 100359222 Rabbit
- Omim: 159552 Human
- SwissProt: Q8HYS5 Dog
- SwissProt: Q07820 Human
- Unigene: 632486 Human
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别名
- Bcl 2 related protein EAT/mcl1 antibody
- Bcl-2-like protein 3 antibody
- Bcl-2-related protein EAT/mcl1 antibody
see all
图片
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All lanes : Anti-MCL1 antibody (ab28147) at 1/500 dilution
Lane 2 : HeLa whole cell lysate
Lane 3 : 3T3 whole cell lysate
Lane 4 : PC-12 whole cell lysate
Lane 5 : MDCK whole cell lysate
Lane 6 : RK-13 whole cell lysate
Predicted band size: 42-43 kDa -
ab28147 staining MCL1 in human breast cancer tissue cells by Immunohistochemistry
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ab28147 staining MCL1 in human HeLa cells by Immunocytochemistry/ immunofluorescence.
实验方案
数据表及文件
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Datasheet download
文献 (8)
ab28147 被引用在 8 文献中.
- Thus YJ et al. Inhibition of casein kinase 2 sensitizes mantle cell lymphoma to venetoclax through MCL-1 downregulation. Haematologica 108:797-810 (2023). PubMed: 36226498
- Yu B et al. Androgen receptor splicing variant 7 (ARV7) inhibits docetaxel sensitivity by inactivating the spindle assembly checkpoint. J Biol Chem 296:100276 (2021). PubMed: 33428943
- Rad S M AH et al. Promoter choice: Who should drive the CAR in T cells? PLoS One 15:e0232915 (2020). PubMed: 32706785
- Kulikov AA et al. Impaired postnatal development of the hippocampus of Krushinsky-Molodkina rats genetically prone to audiogenic seizures. Epilepsy Behav 113:107526 (2020). PubMed: 33161330
- Li X et al. Knockdown of lncRNA CCAT1 enhances sensitivity of paclitaxel in prostate cancer via regulating miR-24-3p and FSCN1. Cancer Biol Ther 21:452-462 (2020). PubMed: 32089062
- Jin X et al. MicroRNA-105 promotes epithelial-mesenchymal transition of nonsmall lung cancer cells through upregulating Mcl-1. J Cell Biochem 120:5880-5888 (2019). PubMed: 30317672
- Cui Y et al. Upregulation of microRNA-383 inhibits the proliferation, migration and invasion of colon cancer cells. Oncol Lett 15:1184-1190 (2018). PubMed: 29399173
- Loriot Y et al. Radiosensitization by a novel Bcl-2 and Bcl-XL inhibitor S44563 in small-cell lung cancer. Cell Death Dis 5:e1423 (2014). WB ; Human . PubMed: 25232677