重组Anti-mCherry抗体[EPR20579] (ab213511)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20579] to mCherry
- Suitable for: IP, WB
- Reacts with: Species independent
Related conjugates and formulations
概述
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产品名称
Anti-mCherry抗体[EPR20579]
参阅全部 mCherry 一抗 -
描述
兔单克隆抗体[EPR20579] to mCherry -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Species independent -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: 293T cells transfected with a His-tagged mCherry whole cell lysate. IP: 293T cells transfected with a His-tagged mCherry whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20579 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab213511于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP | (2) |
1/20.
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WB | (2) |
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 26 kDa).
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说明 |
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IP
1/20. |
WB
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 26 kDa). |
靶标
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相关性
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
图片
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All lanes : Anti-mCherry antibody [EPR20579] (ab213511) at 1/1000 dilution
Lane 1 : 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty expression vector whole cell lysate
Lane 2 : 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged mCherry whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-mCherry antibody [EPR20579] (ab213511) at 1/1000 dilution + Green fluorescent protein (GFP) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Green fluorescent protein (GFP) contains aa1-238 with His-tag.
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mCherry was immunoprecipitated from 0.35 mg of 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged mCherry whole cell lysate with ab213511 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab213511 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1: 293T transfected with a His-tagged mCherry whole cell lysate 10 µg (Input).
Lane 2: ab213511 IP in 293T transfected with a His-tagged mCherry whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213511 in 293T transfected with a His-tagged mCherry whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (21)
ab213511 被引用在 21 文献中.
- Certel SJ et al. A conditional glutamatergic synaptic vesicle marker for Drosophila. G3 (Bethesda) 12:N/A (2022). PubMed: 35100385
- Yan Y et al. Receptor-interacting protein kinase 2 (RIPK2) stabilizes c-Myc and is a therapeutic target in prostate cancer metastasis. Nat Commun 13:669 (2022). PubMed: 35115556
- Yang Y et al. Transcription factors ZmNF-YA1 and ZmNF-YB16 regulate plant growth and drought tolerance in maize. Plant Physiol 190:1506-1525 (2022). PubMed: 35861438
- Johnson JL et al. Differential dysregulation of granule subsets in WASH-deficient neutrophil leukocytes resulting in inflammation. Nat Commun 13:5529 (2022). PubMed: 36130971
- Jin X et al. RAB7 activity is required for the regulation of mitophagy in oocyte meiosis and oocyte quality control during ovarian aging. Autophagy 18:643-660 (2022). PubMed: 34229552