Anti-mCherry抗体(ab183628)
Key features and details
- Rabbit polyclonal to mCherry
- Suitable for: ICC/IF, IHC - Wholemount, IP, WB
- Reacts with: Species independent
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-mCherry抗体
参阅全部 mCherry 一抗 -
描述
兔多克隆抗体to mCherry -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC - Wholemount, IP, WBmore details -
种属反应性
与反应: Species independent -
免疫原
Recombinant full length protein corresponding to mCherry.
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阳性对照
- IHC-Wm: 2 days-post-fertilization zebrafish embryos . WB: Full length recombinant mCherry. mCherry-tagged protein expressing HEK-293T cell lysate. ICC/IF: mCherry fusion protein-transfected HeLa cells IP: HEK-293T
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183628于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (1) |
1/100 - 1/1000.
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IHC - Wholemount |
1/100 - 1/500.
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IP | (2) |
1/100 - 1/500.
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
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说明 |
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ICC/IF
1/100 - 1/1000. |
IHC - Wholemount
1/100 - 1/500. |
IP
1/100 - 1/500. |
WB
1/1000 - 1/10000. Predicted molecular weight: 27 kDa. |
靶标
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相关性
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
图片
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RABV and mGluR2 are internalized into cells and transported together in early and late endosomes.
N2a (Mouse neuroblastoma cells) cells were stained by using the Tyramide Signal Amplification immunofluorescent method. N2a cells infected with ERA-N-mCherry for 20 minutes at 37°C were used to perform immunofluorescence staining for RABV antigen (red), mGluR2 (green), Rab5 or Rab7 (purple), and the cell nuclei (blue). Co-localization of the RABV-mGluR2 complex with Rab5 (E, F).
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Whole mount immunohistochemical analysis of 2 days-post-fertilization zebrafish embryos labeling mCherry-expressing cells with ab183628 at 1/100 dilution.
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All lanes : Anti-mCherry antibody (ab183628) at 1/2000 dilution
Lane 1 : Full length recombinant mCherry at 0.005 µg
Lane 2 : Full length recombinant mCherry at 0.01 µg
Lane 3 : Full length recombinant mCherry at 0.02 µg
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 27 kDa -
All lanes : Anti-mCherry antibody (ab183628) at 1/5000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate/extract
Lane 2 : Whole cell lysate/extract of mCherry-tagged protein expressing HEK-293T cells
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa -
Immunoprecipitation analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate/extract using ab183628 at a 1/3000 dilution.
Lane 1: 20 μg whole cell lysate/extract of Cherry protein-expressing HEK-293T cells.
Lane 2: Control with 2.5 μg of pre-immune rabbit IgG.
Lane 3: Immunoprecipitation of mCherry protein with 2.5 μg of ab183628.7.5% SDS-PAGE. An anti-rabbit IgG (HRP) was used as a secondary reagent.
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Immunofluorescent analysis of mCherry fusion protein-transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. Cells were fixed with 4% paraformaldehyde for 30 minutes at room temperature and then permeabilized with 0.1% Triton X-100 for 1 minute. mCherry (green) was labeled using ab183628 at a dilution of 1/500.
Red: mCherry fusion protein is expressed in the transfected cell.
Blue: Hoechst 33342 staining. -
Immunofluorescent analysis of mCherry fusion protein-transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. Cells were fixed with 4% paraformaldehyde for 30 minutes at room temperature and then permeabilized with 0.1% Triton X-100 for 1 minute. mCherry (green) was labeled using ab183628 at a dilution of 1/500.
Red: mCherry fusion protein is expressed in the transfected cell.
Blue: Hoechst 33342 staining.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (68)
ab183628 被引用在 68 文献中.
- Brandimarti R et al. The US9-Derived Protein gPTB9TM Modulates APP Processing Without Targeting Secretase Activities. Mol Neurobiol 60:1811-1825 (2023). PubMed: 36576708
- Pannek A et al. Dynamics of intracellular neonatal Fc receptor-ligand interactions in primary macrophages using biophysical fluorescence techniques. Mol Biol Cell 33:ar6 (2022). PubMed: 34731029
- Zhang X et al. Exostosin glycosyltransferase 1 reduces porcine reproductive and respiratory syndrome virus infection through proteasomal degradation of nsp3 and nsp5. J Biol Chem 298:101548 (2022). PubMed: 34971707
- Ferrandiz N et al. Endomembranes promote chromosome missegregation by ensheathing misaligned chromosomes. J Cell Biol 221:N/A (2022). PubMed: 35486148
- Saha B et al. Interactomic analysis reveals a homeostatic role for the HIV restriction factor TRIM5α in mitophagy. Cell Rep 39:110797 (2022). PubMed: 35545034