MCF7核extract裂解物(H2O2 post treated) (ab14863)
概述
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产品名称
MCF7核extract裂解物(H2O2 post treated)
参阅全部 MCF7 细胞裂解液 -
常规说明
Post-collection treatment consists in exposing the nuclear extract to a final concentration of 200 mM H2O2 for 20 min on ice. The protein content has been determined by a Bradford-based assay.
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.50
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading...
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研究领域
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背景
MCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin.
图片
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Each lot has been tested for Sp1 activity by using the TransAM™ Sp1 Kit. The signal intensity for Sp1 activity in each lot is compared to the signal intensity obtained with H2O2-post treated extracts from MCF-7 cells. Once the signals are blanked, the ratio between the signals from untreated extracts over treated extracts used at 5
µ g/well must be above 4.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab14863 尚未被引用在任何文献中。