Anti-MCEE抗体[OTI1B5] (ab236397)
Key features and details
- Mouse monoclonal [OTI1B5] to MCEE
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-MCEE抗体[OTI1B5] -
描述
小鼠单克隆抗体[OTI1B5] to MCEE -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human MCEE. Produced in E.coli (NP_115990).
Database link: Q96PE7 -
阳性对照
- WB: pCMV6-ENTRY MCEE cDNA transfected HEK-293T cell lysate. IHC-P: Human colon adenocarcinoma, pancreas carcinoma, endometrium adenocarcinoma, prostate, prostate adenocarcinoma and bladder tissue.
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常规说明
Clone OTI1B5 (formerly 1B5).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant. -
克隆
单克隆 -
克隆编号
OTI1B5 -
同种型
IgG2b
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab236397于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000. Predicted molecular weight: 19 kDa.
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|
IHC-P |
1/150.
Heat-induced epitope retrieval by Tris-EDTA, pH 8.0. |
说明 |
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WB
1/2000. Predicted molecular weight: 19 kDa. |
IHC-P
1/150. Heat-induced epitope retrieval by Tris-EDTA, pH 8.0. |
靶标
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疾病相关
Defects in MCEE are a cause of methylmalonyl-CoA epimerase deficiency (MCEE deficiency) [MIM:251120]; also known as methylmalonyl-CoA racemase deficiency or methylmalonic aciduria type 3. MCEE deficiency is an autosomal recessive inborn error of amino acid metabolism, involving valine, threonine, isoleucine and methionine. This organic aciduria may present in the neonatal period with life-threatening metabolic acidosis, hyperammonemia, feeding difficulties, pancytopenia and coma. -
序列相似性
Belongs to the glyoxalase I family. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 84693 Human
- Omim: 608419 Human
- SwissProt: Q96PE7 Human
- Unigene: 94949 Human
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别名
- DL methylmalonyl CoA racemase antibody
- DL-methylmalonyl-CoA racemase antibody
- EC 5.1.99.1 antibody
see all
图片
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All lanes : Anti-MCEE antibody [OTI1B5] (ab236397) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY MCEE cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 19 kDa -
Paraffin-embedded human bladder tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.
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Paraffin-embedded human prostate carcinoma tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.
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Paraffin-embedded human prostate tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.
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Paraffin-embedded human endometrium adenocarcinoma tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.
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Paraffin-embedded human pancreas carcinoma tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.
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Paraffin-embedded human colon adenocarcinoma tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab236397 尚未被引用在任何文献中。