MBD1多肽(ab4918)
Key features and details
- Suitable for: Blocking
描述
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产品名称
MBD1多肽
参阅全部 MBD1 蛋白酶 -
无动物成分
No -
性质
Synthetic
技术指标
Our Abpromise guarantee covers the use of ab4918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Blocking
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形式
Liquid -
补充说明
This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and human MBD 1, catalog ab2846. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~ 70-fold) for competitive inhibition of antibody-protein binding reactions.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
常规信息
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别名
- CXXC 3
- CXXC-type zinc finger protein 3
- CXXC3
see all -
功能
Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters. -
组织特异性
Widely expressed. -
序列相似性
Contains 3 CXXC-type zinc fingers.
Contains 1 MBD (methyl-CpG-binding) domain. -
结构域
The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions. -
翻译后修饰
Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab4918 被引用在 1 文献中.
- Cerf A et al. Single DNA Molecule Patterning for High-Throughput Epigenetic Mapping. Anal Chem : (2011). PubMed: 21981444