Key features and details
- Rabbit polyclonal to Maxi Potassium channel alpha/SLO
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
产品名称Anti-Maxi Potassium channel alpha/SLO抗体
参阅全部 Maxi Potassium channel alpha/SLO 一抗
描述兔多克隆抗体to Maxi Potassium channel alpha/SLO
特异性Detects Maxi K+ alpha from Human tissues as well as recombinant Human protein.
种属反应性与反应: Mouse, Rat, Human, Pig
预测可用于: Rabbit, Chicken, Cow, Dog, Caenorhabditis elegans, Rhesus monkey
This product was previously labelled as Maxi Potassium channel alpha
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
纯度Immunogen affinity purified
Primary antibody说明Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open.
功能Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).
组织特异性Widely expressed. Except in myocytes, it is almost ubiquitously expressed.
疾病相关Generalized epilepsy and paroxysmal dyskinesia
序列相似性Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa1.1/KCNMA1 sub-subfamily.
Contains 1 RCK N-terminal domain.
结构域The S0 segment is essential for the modulation by the accessory beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4.
The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor.
The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium.
The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel. It includes binding sites for Ca(2+) and Mg(2+).
The calcium bowl constitutes one of the Ca(2+) sensors and probably acts as a Ca(2+)-binding site. There are however other Ca(2+) sensors regions required for activation of the channel.
The heme-binding motif mediates inhibition of channel activation by heme. Carbon monoxide-bound heme leads to increased channel activation.
翻译后修饰Phosphorylated (Probable). Phosphorylation by kinases such as PKA and/or PKG. In smooth muscles, phosphorylation affects its activity.
Palmitoylation by ZDHHC22 and ZDHHC23 within the intracellular linker between the S0 and S1 transmembrane domains regulates localization to the plasma membrane. Depalmitoylated by LYPLA1 and LYPLAL1, leading to retard exit from the trans-Golgi network.
- Information by UniProt
- subfamily M subunit alpha-1 antibody
- BK channel antibody
- BKCA alpha antibody
ab3586 被引用在 5 文献中.
- Khan N et al. BK channels regulate extracellular Tat-mediated HIV-1 LTR transactivation. Sci Rep 9:12285 (2019). PubMed: 31439883
- Ramírez A et al. Calcium-activated potassium channels as potential early markers of human cervical cancer. Oncol Lett 15:7249-7254 (2018). PubMed: 29725443
- Schlüter T et al. miR-96 is required for normal development of the auditory hindbrain. Hum Mol Genet 27:860-874 (2018). PubMed: 29325119
- Mobasheri A et al. Characterization of a stretch-activated potassium channel in chondrocytes. J Cell Physiol 223:511-8 (2010). IHC-P ; Horse . PubMed: 20162564
- Floyd RV et al. Morphology, calcium signaling and mechanical activity in human ureter. J Urol 180:398-405 (2008). Human . PubMed: 18495171