重组Anti-Matrin 3抗体[EPR10635(B)] (ab151714)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10635(B)] to Matrin 3
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Matrin 3抗体[EPR10635(B)]
参阅全部 Matrin 3 一抗 -
描述
兔单克隆抗体[EPR10635(B)] to Matrin 3 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HEK293T, K562, HeLa and HepG2 whole cell lysate (ab7900); Human brain tissue, Human colon tissue and Human breast carcinoma tissue; Mouse and rat liver tissue, Mouse and Rat brain tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR10635(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab151714于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/220.
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WB | (2) |
1/10000 - 1/50000. Predicted molecular weight: 95 kDa.
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
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ICC/IF | (1) |
1/250 - 1/500.
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说明 |
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Flow Cyt (Intra)
1/220. |
WB
1/10000 - 1/50000. Predicted molecular weight: 95 kDa. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
ICC/IF
1/250 - 1/500. |
靶标
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功能
May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs. -
疾病相关
Defects in MATR3 are the cause of myopathy distal type 2 (MPD2) [MIM:606070]; also called vocal cord and pharyngeal dysfunction with distal myopathy (VCPDM). MPD2 is a muscular disorder characterized by distal weakness, with onset in hands and feet, associated with vocal cord and pharyngeal weakness causing a nasal voice and swallowing disorders. -
序列相似性
Contains 1 matrin-type zinc finger.
Contains 2 RRM (RNA recognition motif) domains. -
细胞定位
Nucleus matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 9782 Human
- Entrez Gene: 17184 Mouse
- Entrez Gene: 29150 Rat
- Omim: 164015 Human
- SwissProt: P43243 Human
- SwissProt: Q8K310 Mouse
- SwissProt: P43244 Rat
- Unigene: 268939 Human
see all -
别名
- ALS21 antibody
- KIAA0723 antibody
- Matr3 antibody
see all
图片
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ab151714 was shown to react with MATR3 in wild-type HAP1 cells in Western blot with loss of signal observed in a MATR3 knockout cell line. Wild-type HAP1 and MATR3 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab151714 overnight at 4 °C at a 1/10000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2 μg/ml before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
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All lanes : Anti-Matrin 3 antibody [EPR10635(B)] (ab151714) at 1/10000 dilution (purified)
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Blocking/Diluting buffer 5% NFDM/TBST -
Immunohistochemical analysis of paraffin-embedded human colon tissue sections labeling Matrin 3 with purified ab151714 at a dilution of 1/2000 (0.7 μg/ml). ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody. Sections were counterstained with hematoxylin. Antigen retrieval was heat mediated using citrate Buffer, pH 6.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
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Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Matrin 3 with purified ab151714 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/200, 2.5 μg/mL) - Microtubule Marker (Alexa Fluor® 594) at 1/200. DAPI (blue) was used as a nuclear counterstain. Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
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All lanes : Anti-Matrin 3 antibody [EPR10635(B)] (ab151714) at 1/1000 dilution (unpurified)
Lane 1 : HEK293T cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 95 kDa -
Immunohistochemical analysis of Paraffin-embedded rat liver tissue sections labeling Matrin 3 with purified ab151714 at a dilution of 1/2000 (0.7 μg/ml). ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody. Sections were counterstained with hematoxylin. Antigen retrieval was heat mediated using citrate Buffer, pH 6.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
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Immunohistochemical analysis of paraffin-embedded mouse liver tissue sections labeling Matrin 3 with purified ab151714 at a dilution of 1/2000 (0.7 μg/ml). ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody. Sections were counterstained with hematoxylin. Antigen retrieval was heat mediated using citrate Buffer, pH 6.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
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Intracellular Flow Cytometry analysis of 4%paraformaldehyde fixedHepG2 (human hepatocellular carcinoma) cells labeling Matrin 3 with purified ab151714 at a dilution of 1/220. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Matrin 3 with unpurified ab151714 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Matrin 3 with unpurified ab151714 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of HepG2 cells labeling Matrin 3 with unpurified ab151714 at 1/250 dilution.
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Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using unpurified ab151714 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using unpurified ab151714 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using unpurified ab151714 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab151714 被引用在 10 文献中.
- Kazuma Y et al. ILF2 enhances the DNA cytosine deaminase activity of tumor mutator APOBEC3B in multiple myeloma cells. Sci Rep 12:2278 (2022). PubMed: 35145187
- Hayes LR et al. C9orf72 arginine-rich dipeptide repeat proteins disrupt karyopherin-mediated nuclear import. Elife 9:N/A (2020). PubMed: 32119645
- Ramesh N et al. RNA-recognition motif in Matrin-3 mediates neurodegeneration through interaction with hnRNPM. Acta Neuropathol Commun 8:138 (2020). PubMed: 32811564
- Pandya-Jones A et al. A protein assembly mediates Xist localization and gene silencing. Nature 587:145-151 (2020). PubMed: 32908311
- Sutherland JM et al. RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis. J Cell Physiol 233:3262-3273 (2018). PubMed: 28884822
- Malik AM et al. Matrin 3-dependent neurotoxicity is modified by nucleic acid binding and nucleocytoplasmic localization. Elife 7:N/A (2018). WB, ICC . PubMed: 30015619
- Boehringer A et al. ALS Associated Mutations in Matrin 3 Alter Protein-Protein Interactions and Impede mRNA Nuclear Export. Sci Rep 7:14529 (2017). PubMed: 29109432
- Xu G et al. Vulnerability of newly synthesized proteins to proteostasis stress. J Cell Sci 129:1892-901 (2016). PubMed: 27026526
- Rayaprolu S et al. Heterogeneity of Matrin 3 in the developing and aging murine central nervous system. J Comp Neurol 524:2740-52 (2016). PubMed: 26878116
- Li Y et al. Immunoprecipitation and mass spectrometry defines an extensive RBM45 protein-protein interaction network. Brain Res 1647:79-93 (2016). PubMed: 26979993