Key features and details
- Rabbit polyclonal to Mast Cell Tryptase
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
产品名称Anti-Mast Cell Tryptase抗体
参阅全部 Mast Cell Tryptase 一抗
描述兔多克隆抗体to Mast Cell Tryptase
经测试应用适用于: WB, Flow Cytmore details
Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 68-97 of Human Mast Cell Tryptase (NP_003285).
- 293 cell lysate and 293 cells
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
存储溶液Preservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified through a Protein A column, followed by peptide affinity purification.
Our Abpromise guarantee covers the use of ab129525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 30 kDa.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
相关性Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation.
细胞定位Secreted. Note: released from the secretory granules upon mast cell activation.
- alpha II antibody
- Lung tryptase antibody
- Mast cell alpha II tryptase antibody
Anti-Mast Cell Tryptase antibody (ab129525) at 1/100 dilution + 293 cell lysate at 35 µg
Predicted band size: 30 kDa
ab129525, at a dilution of 1/10 (right histogram), compared to a negative control cell (left histogram) staining Mast Cell Tryptase in 293 cells by Flow Cytometry. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.