Anti-Mast Cell Tryptase抗体(ab129525)
Key features and details
- Rabbit polyclonal to Mast Cell Tryptase
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Mast Cell Tryptase抗体
参阅全部 Mast Cell Tryptase 一抗 -
描述
兔多克隆抗体to Mast Cell Tryptase -
宿主
Rabbit -
经测试应用
适用于: WB, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 68-97 of Human Mast Cell Tryptase (NP_003285).
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阳性对照
- 293 cell lysate and 293 cells
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified through a Protein A column, followed by peptide affinity purification. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab129525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/100 - 1/500. Predicted molecular weight: 30 kDa. | |
| Flow Cyt | 1/10 - 1/50. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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靶标
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相关性
Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation. -
细胞定位
Secreted. Note: released from the secretory granules upon mast cell activation. -
数据库链接
- Entrez Gene: 7177 Human
- Omim: 191080 Human
- SwissProt: Q15661 Human
- Unigene: 405479 Human
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别名
- alpha II antibody
- Lung tryptase antibody
- Mast cell alpha II tryptase antibody
see all
图片
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Western blot - Anti-Mast Cell Tryptase antibody (ab129525)Anti-Mast Cell Tryptase antibody (ab129525) at 1/100 dilution + 293 cell lysate at 35 µg
Predicted band size: 30 kDa -
Flow Cytometry - Anti-Mast Cell Tryptase antibody (ab129525)ab129525, at a dilution of 1/10 (right histogram), compared to a negative control cell (left histogram) staining Mast Cell Tryptase in 293 cells by Flow Cytometry. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
文献 (0)
ab129525 尚未被引用在任何文献中。
