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Immunology Innate Immunity Macrophage / Inflamm.

Anti-Mast Cell Tryptase抗体[AA1] (ab2378)

  • Datasheet
  • SDS
Reviews (33)Q&A (20)References (82)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
  • Western blot - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)

Key features and details

  • Mouse monoclonal [AA1] to Mast Cell Tryptase
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Mast Cell Tryptase抗体[AA1]
    参阅全部 Mast Cell Tryptase 一抗
  • 描述

    小鼠单克隆抗体[AA1] to Mast Cell Tryptase
  • 宿主

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • 免疫原

    Full length native protein (purified) corresponding to Human Mast Cell Tryptase. Human mast cell tryptase was purified from lung tissue by high salt extraction, ammonium sulphate precipitation, octyl Sepharose and heparin-agarose chromatography. (PMID 2253091)

  • 阳性对照

    • WB: Human lung, tonsil and skin tissue lysate. IHC-P: Human skin tissue.
  • 常规说明

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 纯度

    Tissue culture supernatant
  • 克隆

    单克隆
  • 克隆编号

    AA1
  • 骨髓瘤

    unknown
  • 同种型

    IgG1
  • 轻链类型

    unknown
  • 研究领域

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Mast Cell Tryptase protein (denatured) (ab134529)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Cat
Dog
Monkey
应用 Ab评论 说明
WB (4)
1/100 - 1/500. Detects a band of approximately 35-37 kDa (predicted molecular weight: 31 kDa).

We recommend using 3% milk as the blocking agent for Western blot.

IHC-P (18)
1/200000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
WB
1/100 - 1/500. Detects a band of approximately 35-37 kDa (predicted molecular weight: 31 kDa).

We recommend using 3% milk as the blocking agent for Western blot.

IHC-P
1/200000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性

    Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation.
  • 细胞定位

    Secreted. Note: released from the secretory granules upon mast cell activation.
  • 数据库链接

    • Entrez Gene: 7177 Human
    • Entrez Gene: 100503895 Mouse
    • Entrez Gene: 54271 Rat
    • Omim: 191080 Human
    • SwissProt: Q15661 Human
    • SwissProt: Q02844 Mouse
    • SwissProt: P27435 Rat
    • Unigene: 405479 Human
    • Unigene: 3301 Mouse
    • Unigene: 10699 Rat
    see all
  • 别名

    • alpha II antibody
    • Lung tryptase antibody
    • Mast cell alpha II tryptase antibody
    • Mast cell beta I tryptase antibody
    • Mast cell protease 7 antibody
    • Mast cell protease II antibody
    • MCP 7 antibody
    • Pituitary tryptase antibody
    • Skin tryptase antibody
    • TPS 1 antibody
    • TPS1 antibody
    • TPS2 antibody
    • TPSAB1 antibody
    • TPSAB1 protein antibody
    • TPSB1 antibody
    • Tryptase 1 antibody
    • Tryptase alpha 1 antibody
    • tryptase alpha I included antibody
    • Tryptase alpha II antibody
    • tryptase alpha II included antibody
    • tryptase alpha included antibody
    • tryptase alpha/beta 1 antibody
    • Tryptase beta 1 antibody
    • tryptase beta I included antibody
    • Tryptase I antibody
    • tryptase I included antibody
    • Tryptase III antibody
    • Tryptase skin antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)

    IHC image of Mast Cell Tryptase staining in Human Normal Skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. The section was then incubated with ab2378, 1/200,000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
    Western blot - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
    Lanes 1-3 : Anti-Mast Cell Tryptase antibody [AA1] (ab2378) at 1/100 dilution
    Lanes 4-6 : Anti-Mast Cell Tryptase antibody [AA1] (ab2378) at 1/500 dilution

    Lanes 1 & 4 : Human lung normal tissue lysate - total protein (40 - 65 years)
    Lanes 2 & 5 : Human tonsil normal tissue lysate - total protein
    Lanes 3 & 6 : Human skin tissue lysate - total protein

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 31 kDa


    Exposure time: 2 minutes


    Mast Cell Tryptase contains an 18-residue signal sequence and a 12-residue activation peptide. The protein also has two potential glycosylation sites (Swissprot data). These post-translational modifications might explain the banding pattern observed. Abcam used 5% milk in TBS-T as a blocking agent for this blot. We found that this blocking agent removed more non-specific bands than BSA.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)Meyer at al PLoS One. 2013;8(2):e56773. doi: 10.1371/journal.pone.0056773. Epub 2013 Feb 22. Fig 2. Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/

    Tryptase-stained skin biopsy.

    Skin biopsy in CUrt patient stained for tryptase (ab2378, red) at baseline at low (10×, upper panels) and high (60×, lower panels) magnification and at 15 minutes following cold stimulation time test.

    Skin biopsies were obtained at baseline and 15 minutes after CSTT (5 min) on the challenge site, placed into 10% neutral buffered formalin, embedded in paraffin and cut into 5-µm-thick sections. Local anesthesia was performed just before biopsy and did not delay the timing of the biopsy. Slides for immunohistochemistry were deparaffinized and stained. ab2378 was diluted 1:100 and incubated for 2 hours. A biotinylated undiluted secondary antibody of Goat anti-Mouse SS Link, was incubated for 32 minutes, followed with enzyme conjugate, and Fast Red chromogen.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)This image is courtesy of an Abreview submitted by Dr Tom Donndelinger

    ab2378 staining formalin fixed paraffin-embedded human colon tissue sections cut at 1 micron.

    The section was subjected to heat mediated antigen retrieval and permeabilized in Triton-X prior to blocking in 6% BSA for 2 hours at 24°C.  The primary anitbody was diluted 1/250 and incubated with the sample for 16 hours at 5°C.  The secondary antibody was ab7064 (diluted 1/2000) and the slide was counterstained with DAPI.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)De Martin et al PLoS One. 2014 May 15;9(5):e97592. doi: 10.1371/journal.pone.0097592. eCollection 2014. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    AM distribution in thymic mast cells in cryostat sections of human newborn thymic tissue.

    Sections were incubated with an antibody against tryptase (A, B) using ab2378 at a 1/100 dilution.

    V: blood vessel.

    Briefly, frozen sections (10 µm-thick) were cut with a cryostat at −20°C. Antigens were retrieved by heating the sections previously immersed in Bond Epitope Retrieval solution 1 at 100°C for 30 min. Sections were washed in phosphate buffered saline (PBS) and incubated with the primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)Image is courtesy of an AbReview submitted by Dr Fancois Daubeuf

    ab2378 staining Mast Cell Tryptase in rat Lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)Image is courtesy of an AbReview submitted by Carl Hobbs

    ab2378 staining Mast Cell Tryptase in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/7500 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [AA1] (ab2378)Image courtesy of an AbReview submitted by Dr Francois Daubeuf

    ab2378 staining Mast Cell Tryptase in mouse Lung (LPS inflammation) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with paraformaldehyde and blocked with 4% M.O.M Ig blocking for 1 hour at 20°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/200 in blocking buffer) for 16 hours at 4°C. A Biotin-conjugated Rabbit anti-mouse IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

实验方案

  • Mouse on Mouse staining protocol

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (82)

    发表研究结果有使用 ab2378?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2378 被引用在 82 文献中.

    • Tobío A  et al. Oncogenic D816V-KIT signaling in mast cells causes persistent IL-6 production. Haematologica 105:124-135 (2020). PubMed: 30948489
    • Zhu C  et al. Sini San ameliorates duodenal mucosal barrier injury and low-grade inflammation via the CRF pathway in a rat model of functional dyspepsia. Int J Mol Med 45:53-60 (2020). PubMed: 31746413
    • Wang J  et al. Low immune index correlates with favorable prognosis but with reduced benefit from chemotherapy in gallbladder cancer. Cancer Sci 111:219-228 (2020). PubMed: 31729088
    • Matsubara JA  et al. Retinal Distribution and Extracellular Activity of Granzyme B: A Serine Protease That Degrades Retinal Pigment Epithelial Tight Junctions and Extracellular Matrix Proteins. Front Immunol 11:574 (2020). PubMed: 32318066
    • Natsume C  et al. Fucoxanthin Ameliorates Atopic Dermatitis Symptoms by Regulating Keratinocytes and Regulatory Innate Lymphoid Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32235696
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 18 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mast Cell Tryptase antibody [AA1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6
    Permeabilization
    No
    Specification
    skin
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 08 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mast Cell Tryptase antibody [AA1]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (jejunum)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
    Permeabilization
    Yes - TritonX100 0.5%
    Specification
    jejunum
    Blocking step
    MOM Polymer IHC kit blocking reagent ab269452 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formalin 4%
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 06 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mast Cell Tryptase antibody [AA1]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Monkey Tissue sections (Intestines)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Intestines
    Blocking step
    (agent) for 4 minute(s) · Concentration: 3% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More

    Dr. Li Li

    Verified customer

    提交于 Sep 19 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mast Cell Tryptase antibody [AA1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lung Biopsy)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer, ph=6
    Permeabilization
    No
    Specification
    Lung Biopsy
    Fixative
    Formaldehyde
    Read More

    Dr. Francois Daubeuf

    Verified customer

    提交于 Apr 28 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mast Cell Tryptase antibody [AA1]

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    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Cat Tissue sections (Colon)
    Specification
    Colon
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    提交于 Aug 22 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mast Cell Tryptase antibody [AA1]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Goat Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Colon
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    提交于 Aug 22 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mast Cell Tryptase antibody [AA1]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Chicken Tissue sections (Small intestine)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Small intestine
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    提交于 Aug 22 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mast Cell Tryptase antibody [AA1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Dog Tissue sections (Colon)
    Specification
    Colon
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    提交于 Aug 22 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mast Cell Tryptase antibody [AA1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Human Tissue sections (Tonsil)
    Specification
    Tonsil
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    提交于 Aug 22 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mast Cell Tryptase antibody [AA1]

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    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated
    Sample
    Mouse Tissue sections (Heart)
    Specification
    Heart
    Permeabilization
    No
    Fixative
    10% Formalin
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 30 2014

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    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
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