Anti-Mast Cell Tryptase抗体[AA1] (ab2378)

ab97044 would be a great antibody to test with your primary ab2378.

Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an, da wir bei über 100.000 Produkten gar nicht die logistischen Möglichkeiten haben, neben unserer eigentlichen Produktpallette Teströhrchen zu lagern. Hier bei Abcam verfahren wir so, dass wir einen kostenlosen Ersatz oder eine Gutschrift verschicken, falls ein Antikörper nicht so funktioniert wie auf unserem Datenblatt beschrieben, und er innerhalb der 180Tage Garantiezeit reklamiert wird.


Wir möchten Sie jedoch einladen, unser Abreview-System zu verwenden: Das Abreview-System erlaubt es unseren Kunden, Rezensionen unserer Produkte zu veröffendlichen und dafür Punkte zu erhalten. Diese “Abpoints” können gegen Rabatte beim Kauf weiterer Produkte oder Geschenkgutscheine wie z.B. Amazon.com-Gutscheine eingetauscht werden. Sollten Sie sich dazu entscheiden, dieses Produkt zu kaufen, teilen Sie uns bitte Ihre Ergebnisse mittels eines „Abreview“ mit. Sie können die Rezension durch einen Link direkt auf unserem Produktdatenblatt abschicken.

Unter der folgenden URL können Sie mehr über unser Abreview-System zu erfahren:

https://www.abcam.com/abreviews

Vielen Dank für Ihre Hilfe.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie besprochen, und nach einiger Überlegung,habe ich eine kostenlose Ersatzlieferung mit dem ab2378 Anti-Mast Cell Tryptase antibody [AA1]für Sie in Auftrag gegeben, daSie bereits gute Erfahrungen mit diesem Antikörper gemacht haben.Die Lieferunghat die Referenznummer 123456 und sollteam Freitagbei Ihnen ankommen.

Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Vielen Dank für Ihre Anfrage.

Gerne senden wir Ihnen einen kostenlosen Ersatz. Bitte teilen Sie mir doch mit, wann (ungefähr) Sie den Antikörper gekauft haben, damit ich Ihre Bestellung heraus suchen kann.

Wenn Sie eine "Mouse-on-Mouse" Färbung durchführen möchten, kann es natürlich immer zu einem erhöhten Hintergrund kommen. Ich würde dazu entweder einen Sekundär- AK empfehlen, der nur den Isotyp des Primär- AKerkennt (was den Hintergrund aber nicht komplett verschwinden lässt), oder aber ein speziel fuer diese Zwecke entwickeltes IHC Kit empfehlen:

https://www.abcam.com/Mouse-on-Mouse-Polymer-IHC-Kit-ab127055.html (or use the following: https://www.abcam.com/Mouse-on-Mouse-Polymer-IHC-Kit-ab127055.html).

Ich hoffe, dies hilft Ihnen weiter. Ich freue mich auf Ihre Antwort.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

Thank you very much for your call today and for letting us know about the trouble with these antibodies.
Please keep me updated about the results from repeating the staining, and if the problem persists then I would be happy to send replacements for these antibodies.
I look forward to hearing from you. If you have any questions or if there is anything else that we can do for you, please let me know.

Thank you very much for your interest in ab2378.

To our knowledge, ab2378 has not been tested in Mouse. Therefore, I can offer a discount off a future purchase if you buy ab2378 now, test it inMouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free (PRIMARY ANTIBODY/PROTEIN) OR VALUE OFF OF NEXT ORDER.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab2378 in (Mouse). I will then send a discount code. This code must be issued before purchasing ab2378 so please wait for my reply before ordering.

2. Purchase ab2378 either by phone, fax, or online (www.abcam.com).

3. Test it in Mouse.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any [PRIMARY ANTIBODY /PROTEIN /PRODUCT] ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab2378 turns out to be unsuitable for Mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Thank you for contacting Abcam.

As the Abreview was submitted by a customer, we cannot provide any information as to why they used the conditions that they did. It may be that they were following standard protocols that are in their lab, or it may be that they found that the method they used was the only way to get the antibody working in their hands.

I am sorry that I could not provide any more information on this matter, but please let me know if there is anything else I can help you with.

Thank you for contacting us this morning and reporting the problems encountered in using the anti-Mast Cell Tryptase antibody [AA1] (ab2378) in your staining experiments.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. As discussed over the phone, I have arranged for areplacement antibody to be sent to you of a different lot number. This is of the order number 1117141. I really hope that this improves the results you have been observing.

In order for me to investigate this problem further, would you mind providing me with some additional information? If you wouldn't mind filling out the questionnaire I have attached to this email that would be very helpful. This should only take 5-10 minutes and will provide vital information on thesamples tested and the protocol used. Additionally, if you wouldn't mind sharing the images of the parallel staining you performed?

If you could also ask your colleagues about the lot number their vial was from this would be very helpful. If they could give me an approximate delivery date of their order I can probably track it down for them.

This information will allowme to investigate what may be contributingto thisproblem and initiate any internal testing whichmay be required.

Thank you for yourcooperation. I look forward to receiving your reply.

DISCOUNT CODE: ***
Expiration date: May 31st, 2012

I am very pleased to hear you would like to accept our offer and test ab2378 in mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thank you for your enquiry.

I can confirm that ab2378 Mast Cell Tryptase antibody [AA1] has been purified from tissue culture supernatant.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

We have received extra information about MMC detection.

It is possible that this ab2378 can detect mucosal mast cell (MMC), at least if the mast cells in question are human origin. It is a reasonably safe assumption in most cases that all human mast cells contain tryptase, whether in connective tissue sites or in the mucosa. With other species the situation may be different. However, please bear in mind that MMC expression has not been tested/proven by us.

Chymase (detected by CC1 antibody) on the other hand is selectively (though not exclusively) expressed in mast cells in human connective tissues. There have been a few reports of mast cells that may contain chymase but not tryptase, but there may be questions over the extent to which tryptase detection may have been optimal in the double labelling procedures employed. Using frozen sections or occasionally with cytospins, there is scope for tryptase to be leached from the cells if there is inadequate fixation and this could result in an apparent absence of tryptase staining in such specimens.

I hope this information will be helpful.

Thank you for your enquiry.

All the information we have regarding the product is stated on the datasheet. Unfortunately, we have not tested if the product will detect mucosal mast cell (MMC). However, should you wish to know more about the antibody production and details, the following publications will be helpful.

Walls AF, Bennett AR, McBride HM, Glennie MJ, Holgate ST, Church MK. Production and characterisation of monoclonal antibodies specific for human mast cell tryptase. Clinical and Experimental Allergy 1990;20:581-589.

Djukanovic R, Wilson JW, Britten KM, Wilson SJ, Walls AF, Roche WR, Howarth PH, Holgate ST. Quantitation of mast cells and eosinophils in the bronchial mucosa of symptomatic atopic asthmatics and healthy controls using immunocytochemistry. American Review of Respiratory Disease 1990;142:663-671.

http://www.ncbi.nlm.nih.gov/pubmed/2221594

If there is anything else that I can help you with please do not hesitate to contact me.

Have a nice day!

Thank you for contacting us. Your credit note ID is ***. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Thank you for contacting us.  You may be interested in our Histamine or Mast Cell Tryptase antibodies, such as ab5836, ab43870, ab2378, or ab81703.  Unfortunately, I do not know of any commercial sources of human mast cells.  I am sorry I could not be more helpful, please let me know if you have any additional questions. 

Thank you for contacting us. The concentration on the datasheet is for guideline purpose only. The optimal concentration should be determined by end users themselves because the optimum concentration changes with protocol. We appreciate that you have contacted us with this useful information. We have published this information in frequently asked question which will help other scientists as well. Let me know if I can be of further help. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you very much for your interest in the antibody ab2378. Please note that all Abcam products are for research use only and are not intended for diagnostic, therapeutic or any in vivo use. To our knowledge, ab2378 has not been tested in bovine and the epitope has not been mapped. The homology between the human protein, used as immunogen, and the bovine protein is 76.7% (see attachment). Therefore, I can offer a discount off a future purchase if you buy ab2378 now, test it in Bovine and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Contact Kimera, our exclusive distributor in Turkey, and let them know that you would like to proceed and test ab2378 in Bovine. I will then send a discount code to you and Kimera. This code must be issued before purchasing ab2378 so please wait for my reply before ordering. 2. Purchase ab2378 via Kimera. 3. Test it in bovine. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab2378 turns out to be unsuitable for bovine samples, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

Thank you for contacting Abcam regarding ab2378. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for two vials of ab2378. To check the status of the order please contact our Customer Service team and reference this number. Please note that these free of charge replacement vials are also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Thank you for your call yesterday and for letting us know about the problems with the new vials of ab2378. I checked with the lab, and we would recommend storing the antibody at -20C for longer term storage though the antibody should be stable at 4C for at least a week. I am hoping that the replacement vials sent yesterday will work properly and this is an isolated incident. Please keep me updated with any new information, and let me know if you have any questions.

Thank you for your interest in Abcam products. ab2378, ab955 and ab9018 are mouse monoclonal antibodies. I would recommend using ab97044 as a secondary antibody; https://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Biotin-ab97044.html ab5690, ab103573, ab10558 are rabbit polyclonal antibodies. I would recommend using ab6720; https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-ab6720.html ab53444 is a rat monoclonal antibody. I would recommend using ab6733; https://www.abcam.com/Rat-IgG-secondary-antibody-H-L-ab6733.html To let you know we are running a promotion '4 for 3' on primaries; if you could select 1 more primary antibody then you will be entitled to 8 primary antibodies for the price of 6. Use the discount code and follow the instructions given on website. I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.