Anti-Mast Cell Chymase抗体[CC1] (ab2377)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)](https://www.abcam.cn/ps/products/2/ab2377/Images/ab2377-266736-anti-mast-cell-chymase-antibody-cc1-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)")
Key features and details
- Mouse monoclonal [CC1] to Mast Cell Chymase
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Mast Cell Chymase抗体[CC1]
参阅全部 Mast Cell Chymase 一抗 -
描述
小鼠单克隆抗体[CC1] to Mast Cell Chymase -
宿主
Mouse -
经测试应用
适用于: IHC-Pmore details
不适用于: WB -
种属反应性
与反应: Human
预测可用于: Rat, Pig
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免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Tonsil. IHC-P: FFPE human tonsil tissue sections.
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常规说明
Mast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
纯度
Protein G purified -
Primary antibody说明
Mast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation. -
克隆
单克隆 -
克隆编号
CC1 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (2) |
Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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IHC-P
Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. -
组织特异性
Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions. -
序列相似性
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
细胞定位
Secreted. Cytoplasmic granule. Mast cell granules. - Information by UniProt
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数据库链接
- Entrez Gene: 1215 Human
- Entrez Gene: 25627 Rat
- Omim: 118938 Human
- SwissProt: P23946 Human
- SwissProt: P50339 Rat
- Unigene: 135626 Human
- Unigene: 10182 Rat
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别名
- Alpha-chymase antibody
- Chymase 1 antibody
- Chymase 1 mast cell antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)
IHC image of Mast Cell Chymase staining in human tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2377, 0.2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - Anti-Mast Cell Chymase antibody [CC1] (ab2377)](https://www.abcam.cn/ps/products/2/ab2377/Images/ab2377-266737-anti-mast-cell-chymase-antibody-cc1-western-blot.jpg)
Western blot - Anti-Mast Cell Chymase antibody [CC1] (ab2377)All lanes : Anti-Mast Cell Chymase antibody [CC1] (ab2377) at 1 µg/ml
Lane 1 : Recombinant Human Chymase Protein
Lane 2 :Recombinant Human Mast Cell Tryptase protein (denatured) (ab134529)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsAb2377 recognises recombinant human mast cell chymase. However, endogenous protein is not detected in WB due to low expression, therefore we do not guarantee this antibody in WB.
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab2377 被引用在 28 文献中.
- Atiakshin D et al. Protease profile of normal and neoplastic mast cells in the human bone marrow with special emphasis on systemic mastocytosis. Histochem Cell Biol 155:561-580 (2021). PubMed: 33492488
- Kurihara-Shimomura M et al. Mast cell chymase promotes angiogenesis and lymphangiogenesis mediated by activation of melanoma inhibitory activity gene family members in oral squamous cell carcinoma. Int J Oncol 56:1093-1100 (2020). PubMed: 32319583
- Butts B et al. Reduced Left Atrial Emptying Fraction and Chymase Activation in Pathophysiology of Primary Mitral Regurgitation. JACC Basic Transl Sci 5:109-122 (2020). PubMed: 32140620
- Atiakshin D et al. Mast cell chymase: morphofunctional characteristics. Histochem Cell Biol 152:253-269 (2019). PubMed: 31392409
- Powell PC et al. Chymase uptake by cardiomyocytes results in myosin degradation in cardiac volume overload. Heliyon 5:e01397 (2019). PubMed: 30997426