Key features and details
- Rabbit polyclonal to MARS2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
经测试应用适用于: ICC/IF, WB, IHC-Pmore details
- RT4 cell lysates; Human stomach tissue.
常规说明ab130426 is a mono-specific antibody.
The antibody solution should be gently mixed before use.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab130426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||1/250 - 1/500. Predicted molecular weight: 67 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
序列相似性Belongs to the class-I aminoacyl-tRNA synthetase family.
- Information by UniProt
- mars2 antibody
- Methionine tRNA ligase 2 antibody
- Methionine tRNA ligase 2 mitochondrial antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoskeleton (intermediate filaments) and aggresome. Recommended concentration of ab130426 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-MARS2 antibody (ab130426) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Predicted band size: 67 kDa
ab130426, at a dilution of 1/200, staining MARS2 in paraffin-embedded Human stomach tissue by Immunohistochemistry.