Anti-MARS2抗体(ab130426)

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Key features and details

  • Rabbit polyclonal to MARS2
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-MARS2抗体

  • 描述

    兔多克隆抗体to MARS2

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment: WLGTVLHVAL ECLRVFGTLL QPVTPSLADK LLSRLGVSAS ERSLGELYFL PRFYGHPCPF EGRRLGPETG LLFPRLDQSR TWLVKAHRT, corresponding to amino acids 505-593 of Human MARS2 (UniProt: Q96GW9).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • RT4 cell lysates; Human stomach tissue.

  • 常规说明

    ab130426 is a mono-specific antibody.
    The antibody solution should be gently mixed before use.

性能

应用

Our Abpromise guarantee covers the use of ab130426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 0.25 - 2 µg/ml.
WB 1/250 - 1/500. Predicted molecular weight: 67 kDa.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 序列相似性

    Belongs to the class-I aminoacyl-tRNA synthetase family.

  • 细胞定位

    Mitochondrion matrix.
  • Information by UniProt

  • 数据库链接

    • 别名

      • mars2 antibody
      • Methionine tRNA ligase 2 antibody
      • Methionine tRNA ligase 2 mitochondrial antibody
      • Methionine tRNA ligase antibody
      • Methionine tRNA synthetase 2 antibody
      • Methionine--tRNA ligase antibody
      • Methionyl tRNA synthetase 2 mitochondrial antibody
      • Methionyl tRNA synthetase mitochondrial antibody
      • Methionyl-tRNA synthetase 2 antibody
      • MetRS antibody
      • mitochondrial antibody
      • Mitochondrial methionine tRNA ligase antibody
      • Mitochondrial methionyl tRNA synthetase antibody
      • Mitochondrial methionyl-tRNA synthetase antibody
      • MtMetRS antibody
      • SYMM_HUMAN antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-MARS2 antibody (ab130426)
      Immunocytochemistry/ Immunofluorescence - Anti-MARS2 antibody (ab130426)
      Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoskeleton (intermediate filaments) and aggresome. Recommended concentration of ab130426 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Western blot - Anti-MARS2 antibody (ab130426)
      Western blot - Anti-MARS2 antibody (ab130426)
      All lanes : Anti-MARS2 antibody (ab130426) at 1/250 dilution

      Lane 1 : RT4 cell lysate
      Lane 2 : U251 MG cell lysate
      Lane 3 : Human Plasma lysate
      Lane 4 : Human Liver lysate
      Lane 5 : Human Tonsil lysate

      Predicted band size: 67 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARS2 antibody (ab130426)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARS2 antibody (ab130426)
      ab130426, at a dilution of 1/200, staining MARS2 in paraffin-embedded Human stomach tissue by Immunohistochemistry.

    文献

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