Anti-MARS2抗体(ab130426)
Key features and details
- Rabbit polyclonal to MARS2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MARS2抗体 -
描述
兔多克隆抗体to MARS2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment: WLGTVLHVAL ECLRVFGTLL QPVTPSLADK LLSRLGVSAS ERSLGELYFL PRFYGHPCPF EGRRLGPETG LLFPRLDQSR TWLVKAHRT, corresponding to amino acids 505-593 of Human MARS2 (UniProt: Q96GW9).
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阳性对照
- RT4 cell lysates; Human stomach tissue.
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常规说明
ab130426 is a mono-specific antibody.
The antibody solution should be gently mixed before use.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab130426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. | |
| WB | 1/250 - 1/500. Predicted molecular weight: 67 kDa. | |
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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序列相似性
Belongs to the class-I aminoacyl-tRNA synthetase family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 92935 Human
- Omim: 609728 Human
- SwissProt: Q96GW9 Human
- Unigene: 116602 Human
- Unigene: 744330 Human
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别名
- mars2 antibody
- Methionine tRNA ligase 2 antibody
- Methionine tRNA ligase 2 mitochondrial antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-MARS2 antibody (ab130426)Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoskeleton (intermediate filaments) and aggresome. Recommended concentration of ab130426 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Western blot - Anti-MARS2 antibody (ab130426)All lanes : Anti-MARS2 antibody (ab130426) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Predicted band size: 67 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARS2 antibody (ab130426)ab130426, at a dilution of 1/200, staining MARS2 in paraffin-embedded Human stomach tissue by Immunohistochemistry.
实验方案
数据表及文件
文献 (0)
ab130426 尚未被引用在任何文献中。
