Anti-MARK2 (phospho T595)抗体(ab34751)
Key features and details
- Rabbit polyclonal to MARK2 (phospho T595)
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-MARK2 (phospho T595)抗体
参阅全部 MARK2 一抗 -
描述
兔多克隆抗体to MARK2 (phospho T595) -
宿主
Rabbit -
特异性
Reactivity occurs against human MARK2 pT595 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1 and T568 on MARK4. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment
预测可用于: Mouse, Cow, Dog -
免疫原
Synthetic peptide corresponding to Human MARK2 aa 550-650 (phospho T595).
Database link: Q7KZI7 -
阳性对照
- HEK293 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The product was affinity purified from monospecific antiserum by immunoaffinity purification. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab34751于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Detects a band of approximately 82 kDa (predicted molecular weight: 87 kDa).
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说明 |
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WB
1/500 - 1/3000. Detects a band of approximately 82 kDa (predicted molecular weight: 87 kDa). |
靶标
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功能
Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection. -
组织特异性
High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
细胞定位
Cell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 535197 Cow
- Entrez Gene: 483769 Dog
- Entrez Gene: 13728 Mouse
- SwissProt: Q05512 Mouse
- Unigene: 258986 Mouse
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别名
- ELKL motif kinase 1 antibody
- ELKL motif kinase antibody
- EMK-1 antibody
see all
图片
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All lanes : Anti-MARK2 (phospho T595) antibody (ab34751) at 1/500 dilution
Lane 1 : MW markers
Lane 2 : untransfected HEK293 cell lysate
Lane 3 : HEK293 cells transfected with pCMV-3×Flag-
MARK2 plus pCMV-Flag-PKC-zeta
Lane 4 : HEK293 cells transfected with pCMV-3×Flag-MARK2 only
Predicted band size: 87 kDa
Observed band size: 82 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (5)
ab34751 被引用在 5 文献中.
- Lu YN et al. MARK2 phosphorylates eIF2a in response to proteotoxic stress. PLoS Biol 19:e3001096 (2021). PubMed: 33705388
- Mirza AN et al. Combined inhibition of atypical PKC and histone deacetylase 1 is cooperative in basal cell carcinoma treatment. JCI Insight 2:N/A (2017). Mouse . PubMed: 29093271
- Yadav SK et al. Regulation of aPKC activity by Nup358 dependent SUMO modification. Sci Rep 6:34100 (2016). WB ; Human . PubMed: 27682244
- Tobias IS & Newton AC Protein Scaffolds Control Localized Protein Kinase C? Activity. J Biol Chem 291:13809-22 (2016). PubMed: 27143478
- Wu-Zhang AX et al. Cellular Pharmacology of Protein Kinase M? (PKM?) Contrasts with Its in Vitro Profile: IMPLICATIONS FOR PKM? AS A MEDIATOR OF MEMORY. J Biol Chem 287:12879-85 (2012). WB ; Rat . PubMed: 22378786