Anti-MAP4K6抗体- C-terminal (ab154256)
Key features and details
- Rabbit polyclonal to MAP4K6 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-MAP4K6抗体- C-terminal -
描述
兔多克隆抗体to MAP4K6 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 901-1120 of Human MAP4K6 (Uniprot ID: Q8N4C8).
-
阳性对照
- HepG2 whole cell lysate (ab7900); U87 xenograft; HeLa cells
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab154256于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 150 kDa.
|
|
IHC-P |
1/100 - 1/1000.
|
|
ICC/IF |
1/100 - 1/1000.
|
说明 |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 150 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
-
相关性
MAP4K6 is a serine/threonine kinase belonging to the germinal center kinase (GCK) family. These kinases regulate a wide variety of cellular processes by coupling cell surface receptors to MAPK pathways. This kinase appears to act upstream of the JUN N-terminal pathway and may be involved in brain development. It may also play a role in the response to environmental stress, in cytoskeleton reorganization, cell adhesion, and cell motility. Alternative splicing occurs at this locus and four transcript variants encoding distinct isoforms have been identified. -
细胞定位
Cytoplasm. Cell junction; synapse; postsynaptic cell membrane; postsynaptic density. Cell projection; axon. Cell projection; dendrite. Isoform 4: Golgi apparatus -
数据库链接
- Entrez Gene: 50488 Human
- Omim: 609426 Human
- SwissProt: Q8N4C8 Human
-
别名
- B55 antibody
- GCK family kinase MiNK antibody
- hMINK antibody
see all
图片
-
Anti-MAP4K6 antibody - C-terminal (ab154256) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 150 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded U87 xenograft tissue labeling MAP4K6 with ab154256 at 1/500 dilution.
-
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling MAP4K6 with ab154256 at 1/200 dilution. Lower panel merged with DNA probe.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab154256 被引用在 1 文献中.
- Zhang J et al. LncRNA SNHG14 contributes to proinflammatory cytokine production in rheumatoid arthritis via the regulation of the miR-17-5p/MINK1-JNK pathway. Environ Toxicol 36:2484-2492 (2021). PubMed: 34529319