Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Brain cell culture)
Permeabilization
Yes - 0.05% Tween 20
Specification
Brain cell culture
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Sea Block
Dilution
1/1000
Secondary antibody
Dilution
1/300
Name
Abcam antibody:Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed
Additional data
Additional Notes
The signal obtained from using ab5392 Anti-MAP2 antibody on a rat brain culture was pseudo colored in Green and the nuclei stained Blue with Hoechst . Alexa Fluor 647 secondary (ab150175) was used for its better resolution and lack of autofluorescence.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 21 2020