Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (BRAIN)
Antigen retrieval step
None
Permeabilization
Yes - TRITON 1.5%
Specification
BRAIN
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: GOAT SERUM 5%
Dilution
1/250
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: AB194C
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Francoise Geffroy
Verified customer
提交于 Feb 19 2020