Anti-MAP2抗体(ab5392)
Key features and details
- Chicken polyclonal to MAP2
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat
- Isotype: IgY
概述
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产品名称
Anti-MAP2抗体
参阅全部 MAP2 一抗 -
描述
鸡多克隆抗体to MAP2 -
宿主
Chicken -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Recombinant fragment corresponding to Human MAP2. Mix of recombinant human constructs of projection domain sequences, amino acids 235-1588.
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
Preservative: 0.032% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯化说明
Lipid extraction from egg yolk, followed by ammonium sulphate precipitation. -
克隆
多克隆 -
同种型
IgY -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
Our Abpromise guarantee covers the use of ab5392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | 1/5000 - 1/10000. | |
| WB | 1/20000 - 1/100000. This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands. |
靶标
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功能
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. -
序列相似性
Contains 3 Tau/MAP repeats. -
翻译后修饰
Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. -
细胞定位
Cytoplasm, cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 17756 Mouse
- Entrez Gene: 25595 Rat
- SwissProt: P20357 Mouse
- SwissProt: P15146 Rat
- Unigene: 256966 Mouse
- Unigene: 436793 Mouse
- Unigene: 10484 Rat
- Unigene: 136301 Rat
see all -
别名
- DKFZp686I2148 antibody
- MAP 2 antibody
- MAP dendrite specific antibody
see all
图片
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Immunocytochemistry - Anti-MAP2 antibody (ab5392)Rat E20 cultured cortical neuron-glial cells stained for MAP2 (red) using ab5392 at 1/10000 dilution for ICC/IF. Tau is detected with a mouse monoclonal anti-Tau antibody (green). The nuclear counter stain is DAPI (blue). Overlap of MAP2 and Tau staining results in an orange-yellow color.
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Western blot - Anti-MAP2 antibody (ab5392)All lanes : Anti-MAP2 antibody (ab5392) at 1/50000 dilution
Lane 1 : Adult rat brain lysate
Lane 2 : Embryonic E20 rat brain lysate
Lane 3 : Adult mouse brain lysate -
Immunocytochemistry - Anti-MAP2 antibody (ab5392)ab5392 at a dilution of 1/10000, staining MAP 2 (green) in tissue cultured rat cortical neurons by immunofluorescence. Nuclei are stained blue with Dapi. -
Immunocytochemistry - Anti-MAP2 antibody (ab5392)Rat cortical neurons and glia in mixed tissue culture stained with ab5392 (Chicken antibody to MAP2) (green)(1:30 000), a mouse monoclonal antibody to GFAP (red) and nuclei of all cells stained with Hoechst dye (blue). -
Immunocytochemistry - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous Abreviewab5392 staining MAP2 in mouse neurons by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.4% Triton X-100 prior to blocking in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/10000 and incubated with the sample for 20 hours at 21°C. Alexa fluor® 488 goat polyclonal to chicken IgY, diluted 1/400, was used as the secondary antibody. -
Western blot - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous AbreviewAnti-MAP2 antibody (ab5392) at 1/10000 dilution + Mouse brain lysate - post nuclear supernatant at 10 µg
Secondary
HRP-conjugated rabbit anti-chicken IgY at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 280,70 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
文献 (421)
ab5392 被引用在 421 文献中.
- Roy S et al. Neurogenic tissue nanotransfection in the management of cutaneous diabetic polyneuropathy. Nanomedicine 28:102220 (2020). PubMed: 32422219
- Cho Y et al. Elevated O-GlcNAcylation induces an antidepressant-like phenotype and decreased inhibitory transmission in medial prefrontal cortex. Sci Rep 10:6924 (2020). PubMed: 32332789
- Sharma Y et al. In vitro human stem cell derived cultures to monitor calcium signaling in neuronal development and function. Wellcome Open Res 5:16 (2020). PubMed: 32195361
- Pineda-Ramírez N et al. Antioxidant Properties and Protective Effects of Some Species of the Annonaceae, Lamiaceae, and Geraniaceae Families against Neuronal Damage Induced by Excitotoxicity and Cerebral Ischemia. Antioxidants (Basel) 9:N/A (2020). PubMed: 32244955
- Shimojo M et al. Selective Disruption of Inhibitory Synapses Leading to Neuronal Hyperexcitability at an Early Stage of Tau Pathogenesis in a Mouse Model. J Neurosci 40:3491-3501 (2020). PubMed: 32265258
