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Neuroscience Cell Type Marker Neuron marker Soma marker

Anti-MAP2抗体(ab5392)

  • Datasheet
  • SDS
Reviews (57)Q&A (14)References (444)

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Immunocytochemistry - Anti-MAP2 antibody (ab5392)
  • Western blot - Anti-MAP2 antibody (ab5392)
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
  • Western blot - Anti-MAP2 antibody (ab5392)

Key features and details

  • Chicken polyclonal to MAP2
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat
  • Isotype: IgY

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概述

  • 产品名称

    Anti-MAP2抗体
    参阅全部 MAP2 一抗
  • 描述

    鸡多克隆抗体to MAP2
  • 宿主

    Chicken
  • 经测试应用

    适用于: ICC, WBmore details
  • 种属反应性

    与反应: Mouse, Rat
  • 免疫原

    Recombinant fragment corresponding to Human MAP2. Mix of recombinant human constructs of projection domain sequences, amino acids 235-1588.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • 存储溶液

    Preservative: 0.032% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯化说明

    Lipid extraction from egg yolk, followed by ammonium sulphate precipitation.
  • 克隆

    多克隆
  • 同种型

    IgY
  • 研究领域

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • MAP
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Dendrite marker
    • Stem Cells
    • Neural Stem Cells
    • Neuron Restricted Lineage
    • Signal Transduction
    • Antibodies
    • map2

相关产品

  • Compatible Secondaries

    • Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
    • Goat Anti-Chicken IgY H&L (HRP) (ab6877)
  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)
  • Recombinant Protein

    • Recombinant Human MAP2 protein (ab114686)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
    • Anti-Alpha-synuclein antibody [LB 509] (ab27766)
    • Rat MAP2 peptide (ab32453)
    • Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5392于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC (4)
1/5000 - 1/10000.
WB (5)
1/20000 - 1/100000.

This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands.

说明
ICC
1/5000 - 1/10000.
WB
1/20000 - 1/100000.

This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands.

靶标

  • 功能

    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
  • 序列相似性

    Contains 3 Tau/MAP repeats.
  • 翻译后修饰

    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
  • 细胞定位

    Cytoplasm, cytoskeleton.
  • Target information above from: UniProt accession P11137 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 17756 Mouse
    • Entrez Gene: 25595 Rat
    • SwissProt: P20357 Mouse
    • SwissProt: P15146 Rat
    • Unigene: 256966 Mouse
    • Unigene: 436793 Mouse
    • Unigene: 10484 Rat
    • Unigene: 136301 Rat
    • Unigene: 230443 Rat
    see all
  • 别名

    • DKFZp686I2148 antibody
    • MAP 2 antibody
    • MAP dendrite specific antibody
    • MAP-2 antibody
    • MAP2 antibody
    • MAP2A antibody
    • MAP2B antibody
    • MAP2C antibody
    • Microtubule associated protein 2 antibody
    • Microtubule-associated protein 2 antibody
    • MTAP2_HUMAN antibody
    see all

图片

  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Immunocytochemistry - Anti-MAP2 antibody (ab5392)

    Rat E20 cultured cortical neuron-glial cells stained for MAP2 (red) using ab5392 at 1/10000 dilution for ICC/IF. Tau is detected with a mouse monoclonal anti-Tau antibody (green). The nuclear counter stain is DAPI (blue). Overlap of MAP2 and Tau staining results in an orange-yellow color.

  • Western blot - Anti-MAP2 antibody (ab5392)
    Western blot - Anti-MAP2 antibody (ab5392)
    All lanes : Anti-MAP2 antibody (ab5392) at 1/50000 dilution

    Lane 1 : Adult rat brain lysate
    Lane 2 : Embryonic E20 rat brain lysate
    Lane 3 : Adult mouse brain lysate
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    ab5392 at a dilution of 1/10000, staining MAP 2 (green) in tissue cultured rat cortical neurons by immunofluorescence. Nuclei are stained blue with Dapi.
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Rat cortical neurons and glia in mixed tissue culture stained with ab5392 (Chicken antibody to MAP2) (green)(1:30 000), a mouse monoclonal antibody to GFAP (red) and nuclei of all cells stained with Hoechst dye (blue).
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Immunocytochemistry - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous Abreview
    ab5392 staining MAP2 in mouse neurons by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.4% Triton X-100 prior to blocking in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/10000 and incubated with the sample for 20 hours at 21°C. Alexa fluor® 488 goat polyclonal to chicken IgY, diluted 1/400, was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-MAP2 antibody (ab5392)
    Western blot - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous Abreview
    Anti-MAP2 antibody (ab5392) at 1/10000 dilution + Mouse brain lysate - post nuclear supernatant at 10 µg

    Secondary
    HRP-conjugated rabbit anti-chicken IgY at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 280,70 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

实验方案

  • Recommended IF (ICC) protocol with ab5392

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (444)

发表研究结果有使用 ab5392?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab5392 被引用在 444 文献中.

  • Roy S  et al. Neurogenic tissue nanotransfection in the management of cutaneous diabetic polyneuropathy. Nanomedicine 28:102220 (2020). PubMed: 32422219
  • Cho Y  et al. Elevated O-GlcNAcylation induces an antidepressant-like phenotype and decreased inhibitory transmission in medial prefrontal cortex. Sci Rep 10:6924 (2020). PubMed: 32332789
  • Sharma Y  et al. In vitro human stem cell derived cultures to monitor calcium signaling in neuronal development and function. Wellcome Open Res 5:16 (2020). PubMed: 32195361
  • Pineda-Ramírez N  et al. Antioxidant Properties and Protective Effects of Some Species of the Annonaceae, Lamiaceae, and Geraniaceae Families against Neuronal Damage Induced by Excitotoxicity and Cerebral Ischemia. Antioxidants (Basel) 9:N/A (2020). PubMed: 32244955
  • Shimojo M  et al. Selective Disruption of Inhibitory Synapses Leading to Neuronal Hyperexcitability at an Early Stage of Tau Pathogenesis in a Mouse Model. J Neurosci 40:3491-3501 (2020). PubMed: 32265258
View all Publications for this product

客户评价及客户问答

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1-10 of 71 Abreviews or Q&A

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-MAP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step
None
Sample
Rat Tissue sections (Spinal cord)
Specification
Spinal cord
Permeabilization
Yes - 50% Ethanol 30 min
Fixative
Paraformaldehyde
Read More

Mr. Tom Owen

Verified customer

提交于 Apr 08 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (brain)
Permeabilization
Yes - 1% TritonX in PBS
Specification
brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Feb 23 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brain- hippocampal)
Permeabilization
Yes - 1% TritonX in PBS
Specification
Brain- hippocampal
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Feb 23 2021

Immunocytochemistry abreview for Anti-MAP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (iPSC-NSC)
Permeabilization
Yes - 0.5% triton
Specification
iPSC-NSC
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

提交于 Feb 16 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (iPSCs derived human neurons)
Permeabilization
Yes - 0.5% Triton X100
Specification
iPSCs derived human neurons
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Nov 30 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat primary cortical neurons)
Permeabilization
Yes - 0.5% Triton X100
Specification
Rat primary cortical neurons
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Nov 17 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse cortical culture)
Permeabilization
Yes - 0.2% TritonX-100 in PBS for 3hours along with blocking solution.
Specification
mouse cortical culture
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 28°C
Fixative
Paraformaldehyde
Read More

Mr. Lenin Veeraval

Verified customer

提交于 Nov 11 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (184A1 mammary epithelial)
Permeabilization
Yes - PBS + 0.25% Triton-X
Specification
184A1 mammary epithelial
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Oct 19 2020

Western blot abreview for Anti-MAP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Meriones unguiculatus Cell lysate - whole cell (Brain (Meriones unguiculatus) Mongolian gerbil)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
25 µg
Specification
Brain (Meriones unguiculatus) Mongolian gerbil
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Jul 13 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Brain cell culture)
Permeabilization
Yes - 0.05% Tween 20
Specification
Brain cell culture
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Feb 21 2020

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