Anti-MAP2抗体(ab5392)
Key features and details
- Chicken polyclonal to MAP2
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat
- Isotype: IgY
概述
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产品名称
Anti-MAP2抗体
参阅全部 MAP2 一抗 -
描述
鸡多克隆抗体to MAP2 -
宿主
Chicken -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Recombinant fragment corresponding to Human MAP2. Mix of recombinant human constructs of projection domain sequences, amino acids 235-1588.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
Preservative: 0.032% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯化说明
Lipid extraction from egg yolk, followed by ammonium sulphate precipitation. -
克隆
多克隆 -
同种型
IgY -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | (4) |
1/5000 - 1/10000.
|
| WB | (5) |
1/20000 - 1/100000.
This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands. |
| 说明 |
|---|
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ICC
1/5000 - 1/10000. |
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WB
1/20000 - 1/100000. This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands. |
靶标
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功能
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. -
序列相似性
Contains 3 Tau/MAP repeats. -
翻译后修饰
Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. -
细胞定位
Cytoplasm, cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 17756 Mouse
- Entrez Gene: 25595 Rat
- SwissProt: P20357 Mouse
- SwissProt: P15146 Rat
- Unigene: 256966 Mouse
- Unigene: 436793 Mouse
- Unigene: 10484 Rat
- Unigene: 136301 Rat
see all -
别名
- DKFZp686I2148 antibody
- MAP 2 antibody
- MAP dendrite specific antibody
see all
图片
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Immunocytochemistry - Anti-MAP2 antibody (ab5392)
Rat E20 cultured cortical neuron-glial cells stained for MAP2 (red) using ab5392 at 1/10000 dilution for ICC/IF. Tau is detected with a mouse monoclonal anti-Tau antibody (green). The nuclear counter stain is DAPI (blue). Overlap of MAP2 and Tau staining results in an orange-yellow color.
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Western blot - Anti-MAP2 antibody (ab5392)All lanes : Anti-MAP2 antibody (ab5392) at 1/50000 dilution
Lane 1 : Adult rat brain lysate
Lane 2 : Embryonic E20 rat brain lysate
Lane 3 : Adult mouse brain lysate -
Immunocytochemistry - Anti-MAP2 antibody (ab5392)ab5392 at a dilution of 1/10000, staining MAP 2 (green) in tissue cultured rat cortical neurons by immunofluorescence. Nuclei are stained blue with Dapi. -
Immunocytochemistry - Anti-MAP2 antibody (ab5392)Rat cortical neurons and glia in mixed tissue culture stained with ab5392 (Chicken antibody to MAP2) (green)(1:30 000), a mouse monoclonal antibody to GFAP (red) and nuclei of all cells stained with Hoechst dye (blue). -
Immunocytochemistry - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous Abreviewab5392 staining MAP2 in mouse neurons by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.4% Triton X-100 prior to blocking in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/10000 and incubated with the sample for 20 hours at 21°C. Alexa fluor® 488 goat polyclonal to chicken IgY, diluted 1/400, was used as the secondary antibody. -
Western blot - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous AbreviewAnti-MAP2 antibody (ab5392) at 1/10000 dilution + Mouse brain lysate - post nuclear supernatant at 10 µg
Secondary
HRP-conjugated rabbit anti-chicken IgY at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 280,70 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
数据表及文件
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SDS download
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Datasheet download
文献 (444)
ab5392 被引用在 444 文献中.
- Roy S et al. Neurogenic tissue nanotransfection in the management of cutaneous diabetic polyneuropathy. Nanomedicine 28:102220 (2020). PubMed: 32422219
- Cho Y et al. Elevated O-GlcNAcylation induces an antidepressant-like phenotype and decreased inhibitory transmission in medial prefrontal cortex. Sci Rep 10:6924 (2020). PubMed: 32332789
- Sharma Y et al. In vitro human stem cell derived cultures to monitor calcium signaling in neuronal development and function. Wellcome Open Res 5:16 (2020). PubMed: 32195361
- Pineda-Ramírez N et al. Antioxidant Properties and Protective Effects of Some Species of the Annonaceae, Lamiaceae, and Geraniaceae Families against Neuronal Damage Induced by Excitotoxicity and Cerebral Ischemia. Antioxidants (Basel) 9:N/A (2020). PubMed: 32244955
- Shimojo M et al. Selective Disruption of Inhibitory Synapses Leading to Neuronal Hyperexcitability at an Early Stage of Tau Pathogenesis in a Mouse Model. J Neurosci 40:3491-3501 (2020). PubMed: 32265258