Anti-Maltose Binding蛋白抗体[R29.6] (ab65)

Reviews (2)Q&A (4)References (6)
Western blot - Anti-Maltose Binding Protein antibody [R29.6] (ab65)

    Key features and details

    • Mouse monoclonal [R29.6] to Maltose Binding Protein
    • Suitable for: IP, WB, ChIP
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-Maltose Binding蛋白抗体[R29.6]
      参阅全部 Maltose Binding Protein 一抗

    • 描述

      小鼠单克隆抗体[R29.6] to Maltose Binding蛋白

    • 宿主

      Mouse

    • 经测试应用

      适用于: IP, WB, ChIPmore details

    • 免疫原

      MOS maltose binding protein fusion protein.

    • 常规说明

      MBP is a bacterial protein, which is also used as a fusion protein in expression constructs.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Concentration information loading...

    • 纯度

      Protein A purified

    • 克隆

      单克隆

    • 克隆编号

      R29.6

    • 骨髓瘤

      Sp2/0

    • 同种型

      IgG1

    • 轻链类型

      unknown

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab65于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IP
    Use at an assay dependent concentration.
    WB (1)
    Use at an assay dependent concentration.
    ChIP (1)
    Use at an assay dependent concentration.
    说明
    IP
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration.
    ChIP
    Use at an assay dependent concentration.

    靶标

    • 相关性

      Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.

    • 细胞定位

      Periplasm

    • 别名

      • ECK4026 antibody
      • JW3994 antibody
      • Mal E antibody
      • MalE antibody
      • malJ antibody
      • Maltodextrin binding protein antibody
      • Maltose ABC transporter periplasmic protein antibody
      • Maltose binding periplasmic protein antibody
      • MMBP antibody
      • Periplasmic maltose binding protein antibody
      see all

    图片

    • Western blot - Anti-Maltose Binding Protein antibody [R29.6] (ab65)
      Western blot - Anti-Maltose Binding Protein antibody [R29.6] (ab65)Image from Verhoeven GS et al., PLoS One. 2009 Aug 25;4(8):e6739.Fig S6.; doi:10.1371/journal.pone.0006739; August 25, 2009, PLoS ONE 4(8): e6739.
      Representative western blot detecting Maltose Binding Protein using ab65 at 1.8 µg/ml.

    文献 (6)

    发表研究结果有使用 ab65？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab65 被引用在 6 文献中.

    • Kamennaya NA & Post AF Characterization of cyanate metabolism in marine Synechococcus and Prochlorococcus spp. Appl Environ Microbiol 77:291-301 (2011). PubMed: 21057026
    • Im YJ  et al. Structure and function of the ESCRT-II-III interface in multivesicular body biogenesis. Dev Cell 17:234-43 (2009). PubMed: 19686684
    • Verhoeven GS  et al. Differential bacterial surface display of peptides by the transmembrane domain of OmpA. PLoS One 4:e6739 (2009). WB . PubMed: 19707582
    • Liu X  et al. Whole-genome comparison of Leu3 binding in vitro and in vivo reveals the importance of nucleosome occupancy in target site selection. Genome Res 16:1517-28 (2006). PubMed: 17053089
    • Liu X  et al. DIP-chip: rapid and accurate determination of DNA-binding specificity. Genome Res 15:421-7 (2005). ChIP ; Saccharomyces cerevisiae . PubMed: 15710749
    • Leliveld SR  et al. Apoptin induces tumor-specific apoptosis as a globular multimer. J Biol Chem 278:9042-51 (2003). ICC/IF . PubMed: 12496278

    客户评价及客户问答

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    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-Maltose Binding Protein antibody [R29.6]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Bacteria Recombinant protein (BL-21)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (10%)
    Loading amount
    10 µg
    Specification
    BL-21
    Blocking step
    BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More

    Jun Soung Kwak

    Verified customer

    提交于 Jan 09 2020

    Question

    What is the epitope recognized by product ab65?

    Read More
    Answer

    Unfortunately, the epitope recognized by product ab65 has not yet been mapped.

    Read More

    ChIP abreview for Anti-Maltose Binding Protein antibody [R29.6] - ChIP Grade

     Excellent
    Abreviews
    Application
    ChIP
    Sample
    Saccharomyces cerevisiae Cell (S. cerevisae)
    Specification
    S. cerevisae
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 0 second(s)
    Specification of the cross-linking agent: formaldehyde
    Detection step
    Other
    Positive control
    negative control: MBP-expressing vector in yeast
    Read More

    Mr. Xiao Liu

    Verified customer

    提交于 Aug 11 2005

    Question

    Do you know the gene which encodes the MBP to which these antibodies were raised? I am planning on getting a model where this gene is mutated, and use it as a negative control in my specific experiment.

    Read More
    Answer

    This antibody was raised against a fusion protein and unfortunately we don't have the amino acid sequence.

    Read More

    Question

    Can the anti-MBP (ref # ab65) be used in an ELISA assay? Second, can your company directly attach an HRP or AP conjugate directly to the anti-MBP? If so, how much does this cost?

    Read More
    Answer

    I am afraid that this antibody has not been tested yet in ELISA. We also don't currently provide a conjugation service for our antibodies, however we do supply a range of conjugated secondary antibodies that may be suitable for your needs.

    Read More

    Question

    At what dilution should one use the Anti-MBP (Ref. number: ab65)?

    Read More
    Answer

    The optimal dilution should be determined for each application , but a good start point for Western blots would be 1µg/ml

    Read More

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