Anti-Maltose Binding蛋白抗体[R29.6] (ab65)

Reviews (2)Q&A (4)References (6)

概述

  • 产品名称

    Anti-Maltose Binding蛋白抗体[R29.6]
    参阅全部 Maltose Binding Protein 一抗

  • 描述

    小鼠单克隆抗体[R29.6] to Maltose Binding蛋白

  • 宿主

    Mouse

  • 经测试应用

    适用于: IP, WB, ChIP, ICC/IFmore details

  • 免疫原

    MOS maltose binding protein fusion protein.

  • 常规说明

    MBP is a bacterial protein, which is also used as a fusion protein in expression constructs.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    R29.6

  • 骨髓瘤

    Sp2/0

  • 同种型

    IgG1

  • 轻链类型

    unknown

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab65 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 12496278

靶标

  • 相关性

    Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.

  • 细胞定位

    Periplasm

  • 别名

    • ECK4026 antibody
    • JW3994 antibody
    • Mal E antibody
    • MalE antibody
    • malJ antibody
    • Maltodextrin binding protein antibody
    • Maltose ABC transporter periplasmic protein antibody
    • Maltose binding periplasmic protein antibody
    • MMBP antibody
    • Periplasmic maltose binding protein antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-Maltose Binding Protein antibody [R29.6] (ab65)
    Immunocytochemistry/ Immunofluorescence - Anti-Maltose Binding Protein antibody [R29.6] (ab65)Image from Leliveld SR et al., J Biol Chem. 2003 Mar 14;278(11):9042-51. Epub 2002 Dec 19. Fig 6.; doi: 10.1074/jbc.M210803200; March 14, 2003 The Journal of Biological Chemistry, 278, 9042-9051
    Immunofluorescence analysis of Saos-2 cells, transfected with full-length MBP-Apoptin and MBP-Apoptin(80–121). After fixation, the cells were stained for Maltose Binding Protein using ab65. Nuclear morphology is indicated by DAPI staining.
  • Western blot - Anti-Maltose Binding Protein antibody [R29.6] (ab65)
    Western blot - Anti-Maltose Binding Protein antibody [R29.6] (ab65)Image from Verhoeven GS et al., PLoS One. 2009 Aug 25;4(8):e6739.Fig S6.; doi:10.1371/journal.pone.0006739; August 25, 2009, PLoS ONE 4(8): e6739.
    Representative western blot detecting Maltose Binding Protein using ab65 at 1.8 µg/ml.

文献

This product has been referenced in:

  • Kamennaya NA & Post AF Characterization of cyanate metabolism in marine Synechococcus and Prochlorococcus spp. Appl Environ Microbiol 77:291-301 (2011). Read more (PubMed: 21057026) »
  • Im YJ  et al. Structure and function of the ESCRT-II-III interface in multivesicular body biogenesis. Dev Cell 17:234-43 (2009). Read more (PubMed: 19686684) »
See all 6 Publications for this product

发表研究结果有使用 ab65？请让我们知道，以便我们可以引用本数据表中的参考文章。

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1-6 of 6 Abreviews or Q&A

Western blot abreview for Anti-Maltose Binding Protein antibody [R29.6]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Bacteria Recombinant protein (BL-21)
Gel Running Conditions
Reduced Non-Denaturing (Native) (10%)
Loading amount
10 µg
Specification
BL-21
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Read More

Jun Soung Kwak

Verified customer

提交于 Jan 09 2020

Question

What is the epitope recognized by product ab65?

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Answer

Unfortunately, the epitope recognized by product ab65 has not yet been mapped.

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ChIP abreview for Anti-Maltose Binding Protein antibody [R29.6] - ChIP Grade

 Excellent
Abreviews
Application
ChIP
Sample
Saccharomyces cerevisiae Cell (S. cerevisae)
Specification
S. cerevisae
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Other
Positive control
negative control: MBP-expressing vector in yeast
Read More

Mr. Xiao Liu

Verified customer

提交于 Aug 11 2005

Question

Do you know the gene which encodes the MBP to which these antibodies were raised? I am planning on getting a model where this gene is mutated, and use it as a negative control in my specific experiment.

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Answer

This antibody was raised against a fusion protein and unfortunately we don't have the amino acid sequence.

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Question

Can the anti-MBP (ref # ab65) be used in an ELISA assay? Second, can your company directly attach an HRP or AP conjugate directly to the anti-MBP? If so, how much does this cost?

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Answer

I am afraid that this antibody has not been tested yet in ELISA. We also don't currently provide a conjugation service for our antibodies, however we do supply a range of conjugated secondary antibodies that may be suitable for your needs.

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Question

At what dilution should one use the Anti-MBP (Ref. number: ab65)?

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Answer

The optimal dilution should be determined for each application , but a good start point for Western blots would be 1µg/ml

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