Anti-Malaria ELISA试剂盒(ab178649)
Key features and details
- Sensitivity: 95.9 %
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
概述
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产品名称
Anti-Malaria ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Positive 22 2.8% Positive 22 3.9% Negative 22 5.5% 批次间 样品 n Mean SD CV% Positive 24 3.2% Positive 24 4.8% Negative 24 10.3% -
样品类型
Serum, Hep Plasma, Cit plasma -
检测类型
Indirect -
灵敏度
= 95.9 % -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s anti-Malaria Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG and IgM class antibodies against Malaria in Human serum and plasma.
A 96-well plate has been precoated with Malaria antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG and anti-Human IgM conjugate is added to the wells, which binds to the immobilized Malaria antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Malaria IgG or Malaria IgM sample captured in plate.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover Foil 1 unit IgG Sample Diluent 1 x 100ml Malaria Anti-IgG and Anti-IgM HRP Conjugate 1 x 20ml Malaria Coated Microplate (12 x 8 wells) 1 unit Malaria Cut-off Control 1 x 3ml Malaria Negative Control 1 x 2ml Malaria Positive Control 1 x 2ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
研究领域
图片
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Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
数据表及文件
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SDS download
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Datasheet download
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