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Cell Biology Cell Cycle Cell Division Spindle

Anti-Mad2L2/REV7抗体(ab3630)

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Western blot - Anti-Mad2L2/REV7 antibody (ab3630)

    Key features and details

    • Rabbit polyclonal to Mad2L2/REV7
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-Mad2L2/REV7抗体
      参阅全部 Mad2L2/REV7 一抗
    • 描述

      兔多克隆抗体to Mad2L2/REV7
    • 宿主

      Rabbit
    • 特异性

      This antibody detects a single band just below the 21kD marker, that corresponds to Mad2L2/REV7.

    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Xenopus laevis
    • 免疫原

      Synthetic peptide corresponding to Human Mad2L2/REV7 aa 3-14.
      Sequence:

      TLTRQDLNFGQV


      (Peptide available as ab11254)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

       This product was previously labelled as Mad2L2

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Cell Biology
      • Cell Cycle
      • Cell Division
      • Spindle
      • Cancer
      • Cell cycle
      • Cell division

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Immunizing Peptide (Blocking)

      • Human Mad2L2/REV7 peptide (ab11254)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    应用

    Our Abpromise guarantee covers the use of ab3630 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB 1/500. Detects a band of approximately 20 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human Mad2L2/REV7 peptide (ab11254).

    靶标

    • 功能

      Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.
    • 组织特异性

      Ubiquitously expressed.
    • 序列相似性

      Contains 1 HORMA domain.
    • 细胞定位

      Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm.
    • Target information above from: UniProt accession Q9UI95 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 10459 Human
      • Entrez Gene: 394380 Xenopus laevis
      • Omim: 604094 Human
      • SwissProt: Q9UI95 Human
      • SwissProt: Q8QFR4 Xenopus laevis
      • Unigene: 19400 Human
      • Unigene: 8200 Xenopus laevis
      • 别名

        • Homolog of REV7 S cerevisiae antibody
        • hREV7 antibody
        • MAD2 (mitotic arrest deficient yeast, homolog) like 2 antibody
        • MAD2 homolog antibody
        • MAD2 like 2 antibody
        • MAD2 mitotic arrest deficient like 2 antibody
        • MAD2-like protein 2 antibody
        • MAD2B antibody
        • Mad2l2 antibody
        • MD2L2_HUMAN antibody
        • Mitotic Arrest Deficient 2 L2 antibody
        • Mitotic arrest deficient 2-like protein 2 antibody
        • Mitotic arrest deficient homolog like 2 antibody
        • Mitotic arrest deficient like 2 (yeast) antibody
        • Mitotic arrest deficient yeast homolog antibody
        • Mitotic spindle assembly checkpoint protein MAD2B antibody
        • Polymerase (DNA directed) zeta 2 accessory subunit antibody
        • POLZ2 antibody
        • REV 7 antibody
        • REV7 antibody
        • REV7 homolog antibody
        • Weakly similar to Mitotic MAD2 protein (S cerevisiae) antibody
        see all

      图片

      • Western blot - Anti-Mad2L2/REV7 antibody (ab3630)
        Western blot - Anti-Mad2L2/REV7 antibody (ab3630)
        All lanes : Anti-Mad2L2/REV7 antibody (ab3630) at 1/500 dilution

        Lane 1 : Whole cell extract prepared from Hela cells
        Lane 2 : Nuclear lysates prepared from Hela cells

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

        Predicted band size: 24 kDa



        Western blot with ab3630 at 1/500.

        Secondary: ab6721 at 1/2000.
        Exposure: 30 sec.
        Lysates at 20 µg/lane.

        Lane 1: HeLa Whole Cell extract.
        Lane 2: HeLa Nuclear extract.

      实验方案

      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet
    • 文献 (0)

      发表研究结果有使用 ab3630?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab3630 尚未被引用在任何文献中。

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      Western blot abreview for Anti-Mad2L2/REV7 antibody

      Inconclusive
      Abreviews
      Abreviews
      Application
      Western blot
      Sample
      Zebrafish Tissue lysate - whole (whole larvae)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      100 µg
      Specification
      whole larvae
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More

      Mr. Gregory Weber

      Verified customer

      提交于 Aug 04 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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