Key features and details
- Rabbit polyclonal to Mad2L2/REV7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
纯度Immunogen affinity purified
纯化说明This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab3630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 20 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human Mad2L2/REV7 peptide (ab11254).|
功能Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.
序列相似性Contains 1 HORMA domain.
细胞定位Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm.
- Information by UniProt
- Homolog of REV7 S cerevisiae antibody
- hREV7 antibody
- MAD2 (mitotic arrest deficient yeast, homolog) like 2 antibody
All lanes : Anti-Mad2L2/REV7 antibody (ab3630) at 1/500 dilution
Lane 1 : Whole cell extract prepared from Hela cells
Lane 2 : Nuclear lysates prepared from Hela cells
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Predicted band size: 24 kDa
Western blot with ab3630 at 1/500.
Secondary: ab6721 at 1/2000.
Exposure: 30 sec.
Lysates at 20 µg/lane.
Lane 1: HeLa Whole Cell extract.
Lane 2: HeLa Nuclear extract.