Anti-Mad2L2/REV7抗体(ab3630)
Key features and details
- Rabbit polyclonal to Mad2L2/REV7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Mad2L2/REV7抗体
参阅全部 Mad2L2/REV7 一抗 -
描述
兔多克隆抗体to Mad2L2/REV7 -
宿主
Rabbit -
特异性
This antibody detects a single band just below the 21kD marker, that corresponds to Mad2L2/REV7.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Xenopus laevis
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免疫原
Synthetic peptide corresponding to Human Mad2L2/REV7 aa 3-14.
Sequence:TLTRQDLNFGQV
(Peptide available asab11254) -
常规说明
This product was previously labelled as Mad2L2
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
应用
Our Abpromise guarantee covers the use of ab3630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500. Detects a band of approximately 20 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human Mad2L2/REV7 peptide (ab11254). |
靶标
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功能
Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation. -
组织特异性
Ubiquitously expressed. -
序列相似性
Contains 1 HORMA domain. -
细胞定位
Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 10459 Human
- Entrez Gene: 394380 Xenopus laevis
- Omim: 604094 Human
- SwissProt: Q9UI95 Human
- SwissProt: Q8QFR4 Xenopus laevis
- Unigene: 19400 Human
- Unigene: 8200 Xenopus laevis
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别名
- Homolog of REV7 S cerevisiae antibody
- hREV7 antibody
- MAD2 (mitotic arrest deficient yeast, homolog) like 2 antibody
see all
图片
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Western blot - Anti-Mad2L2/REV7 antibody (ab3630)All lanes : Anti-Mad2L2/REV7 antibody (ab3630) at 1/500 dilution
Lane 1 : Whole cell extract prepared from Hela cells
Lane 2 : Nuclear lysates prepared from Hela cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Predicted band size: 24 kDaWestern blot with ab3630 at 1/500.
Secondary: ab6721 at 1/2000.
Exposure: 30 sec.
Lysates at 20 µg/lane.Lane 1: HeLa Whole Cell extract.
Lane 2: HeLa Nuclear extract.
数据表及文件
文献 (0)
ab3630 尚未被引用在任何文献中。
