Application
Western blot
Sample
Human Cell lysate - whole cell (Colon carcinoma)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
40 µg
Specification
Colon carcinoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: milk 1% TBS Tween
Dilution
1/2000
Secondary antibody
Dilution
1/6000
Name
Non-Abcam antibody was used: mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 06 2016