概述

  • 产品名称
  • 描述
    兔多克隆抗体to MACC1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human MACC1 (internal sequence). MACC1 antibody was raised against a 17 amino acid synthetic peptide near the center of human MACC1
    Database link: Q6ZN28

  • 阳性对照
    • Mouse liver tissue lysate; Human liver tissue IF: A431 cell line

性能

应用

Our Abpromise guarantee covers the use of ab106579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 97 kDa.
IHC-P Use a concentration of 2.5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能
    Acts as a transcription activator for MET and as a key regulator of HGF-MET signaling. Promotes cell motility, proliferation and hepatocyte growth factor (HGF)-dependent scattering in vitro and tumor growth and metastasis in vivo.
  • 组织特异性
    Preferentially expressed in metastasizing tumors.
  • 序列相似性
    Contains 1 SH3 domain.
  • 细胞定位
    Cytoplasm. Nucleus. Mainly found in the cytoplasm in non-metastasizing tumors.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 7A5 antibody
    • MACC1 antibody
    • MACC1_HUMAN antibody
    • Metastasis associated in colon cancer 1 antibody
    • Metastasis-associated in colon cancer protein 1 antibody
    • OTTHUMP00000201891 antibody
    • Putative binding protein 7a5 antibody
    • SH3 domain-containing protein 7a5 antibody
    • SH3BP4L antibody
    see all

图片

  • Western blot analysis of MACC1 expression in human liver tissue lysate with ab106579 at (A) 1 and (B) 2µg/ml.
  • Lane 1 : Anti-MACC1 antibody (ab106579) at 1 µg/ml
    Lane 2 : Anti-MACC1 antibody (ab106579) at 2 µg/ml

    All lanes : Mouse liver tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 97 kDa

  • Immunohistochemical analysis of MACC1 expression in paraffin embedded Human liver tissue, using ab106579 at 2.5 µg/ml.
  • ICC/IF image of ab106579 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106579, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Lane 1 : Anti-MACC1 antibody (ab106579) at 1 µg/ml
    Lane 2 : Anti-MACC1 antibody (ab106579) at 2 µg/ml

    All lanes : Human liver tissue lysate

    Predicted band size: 97 kDa

  • Immunofluorescence of MACC1 in Human Liver cells using ab106579 at 20 ug/ml.

文献

This product has been referenced in:
  • Ma L  et al. Long non-coding RNA XIST promotes cell growth and invasion through regulating miR-497/MACC1 axis in gastric cancer. Oncotarget 8:4125-4135 (2017). WB ; Human . Read more (PubMed: 27911852) »
  • Zhou W  et al. Combination of Endothelial-Monocyte-Activating Polypeptide-II with Temozolomide Suppress Malignant Biological Behaviors of Human Glioblastoma Stem Cells via miR-590-3p/MACC1 Inhibiting PI3K/AKT/mTOR Signal Pathway. Front Mol Neurosci 10:68 (2017). Read more (PubMed: 28348518) »
See all 5 Publications for this product

客户评价及客户问答

Question
Answer

Thank you for contacting us. Your credit note ID is 18744. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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