Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-mTOR (phospho S2448) antibody [EPR426(2)]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH8, 20 min 100C
Sample
Human Tissue sections (breast cancer)
Specification
breast cancer
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako diluent, background reducers
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Leica Refine Goat anti-mouse, anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Additional data

Additional Notes
Immunohistochemistry validated and autometed on Leica Bond III. Various retrieval regimes tested with EDTA giving best signal to background

Abcam user community

Verified customer

提交于 Feb 26 2015

注册