Application
Western blot
Sample
Human Tissue lysate - whole (Placenta)
Loading amount
20 µg
Specification
Placenta
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/2000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/Tween
Secondary antibody
Name
Non-Abcam antibody was used: Peroxidase labeled anti-rabbit IgG (Vector Laborat
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 240 kDa Non-specific: 115, 84 kDa
Additional data
PubMed ID
17463046: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 21 2007