Western blot abreview for Anti-mTOR antibody [Y391] - ChIP Grade

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HUVEC)
Loading amount
45 µg
Specification
HUVEC
Treatment
FGF2 50 ng/ml for 5 minutes
Gel Running Conditions
Reduced Denaturing (gradient 4-15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-tween 20

Secondary antibody

Name
Non-Abcam antibody was used: HRP-conjugated anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: > 250 kDa

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提交于 Sep 17 2008

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