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I am attempting to immunoprecipitate mTOR and known interacting proteins (specifically Raptor and Rictor) with ab32028. I am successfully pulling down mTOR (as evidenced by Western blotting), but it is very inefficient and I am not seeing interacting proteins. Could I receive some advice/assistance on immunoprecipitation with this antibody? Please let me know what other info you would like from me about my protocol.
Asked on Aug 09 2012
Thank you for your reply.
As requested, I am sending you a new vial of mTOR antibody, ab2732 to use in your IP experiments, since the original vial of antibody was not working as well as it should have. Your new order number, for tracking purposes only, is *.
If there is anything else I can help you with, or if you have any issues with the new vial of the antibody, then please let me know.
Abcam Scientific Support
回复于 Aug 09 2012