Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Humen leukemia cells with mCherry in murine bone)
Permeabilization
Yes - 0.1% Tween
Specification
Humen leukemia cells with mCherry in murine bone
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Formalin
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% Tween 5% Goat in PBS
Dilution
1/50
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Alexa Fluor 594 goat anti-Rabbit lgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594 + Gold 1.4nm
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594 + Gold 1.4nm
Additional data
Abcam response
Please note, that the customer has obtained the Abtrial for testing this antibody in IHC-Fr - a not tested yet application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Apr 20 2015