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Immunology Innate Immunity Macrophage / Inflamm.
使用敲除细胞株进行验证重组RabMAb

重组Anti-M6PR (cation independent)抗体[EPR6599] (ab124767)

  • Datasheet
  • SDS
Reviews (6)Q&A (1)References (39)

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Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
  • Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
  • Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
  • Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
  • Flow Cytometry - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
  • OI-RD Scanning - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
  • Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6599] to M6PR (cation independent)
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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概述

  • 产品名称

    Anti-M6PR (cation independent)抗体[EPR6599]
    参阅全部 M6PR (cation independent) 一抗
  • 描述

    兔单克隆抗体[EPR6599] to M6PR (cation independent)
  • 宿主

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Rat
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide within Human M6PR (cation independent) aa 2450 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P11717
    (Peptide available as ab169957)

  • 阳性对照

    • Jurkat, 293T, C6, PC-12, NIH/3T3 and Caco-2 cell lysates; Human papillary carcinoma, thyroid and tonsil tissue; Mouse heart, kidney, colon and spleen tissue; Rat colon tissue . ICC/IF: HAP1 WT and HAP1-IGF2R knockout cells
  • 常规说明

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • 解离常数(KD)

    KD = 3.90 x 10 -12 M
    Learn more about KD
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR6599
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Endosome
    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins

相关产品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab184807)
    • Alexa Fluor® 647 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab198324)
    • Alexa Fluor® 405 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab198515)
    • Alexa Fluor® 594 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab203437)
    • Alexa Fluor® 555 Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab203438)
    • APC Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab225131)
    • PE Anti-M6PR (cation independent) antibody [EPR6599] (ab225132)
    • Anti-M6PR (cation independent) antibody [EPR6599] - BSA and Azide free (ab226090)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunizing Peptide (Blocking)

    • M6PR (cation independent) peptide (ab169957)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Lysosomal Staining Kit - Blue Fluorescence - Cytopainter (ab112135)
    • Lysosomal Staining Kit - Red Fluorescence - Cytopainter (ab112137)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Mouse
Rat
Human
WB
Rat
Human
应用 Ab评论 说明
WB (3)
1/50000 - 1/200000. Detects a band of approximately 300 kDa (predicted molecular weight: 274 kDa).Can be blocked with M6PR (cation independent) peptide (ab169957).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98°C, below boiling, and then let cool for 10-20 min.

See IHC antigen retrieval protocols.

Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF (3)
Use a concentration of 1 µg/ml.
说明
WB
1/50000 - 1/200000. Detects a band of approximately 300 kDa (predicted molecular weight: 274 kDa).Can be blocked with M6PR (cation independent) peptide (ab169957).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98°C, below boiling, and then let cool for 10-20 min.

See IHC antigen retrieval protocols.

Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
Use a concentration of 1 µg/ml.

靶标

  • 功能

    Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
  • 序列相似性

    Belongs to the MRL1/IGF2R family.
    Contains 1 fibronectin type-II domain.
  • 结构域

    Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.
  • 细胞定位

    Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.
  • Target information above from: UniProt accession P11717 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3482 Human
    • Entrez Gene: 16004 Mouse
    • Entrez Gene: 25151 Rat
    • Omim: 147280 Human
    • SwissProt: P11717 Human
    • SwissProt: Q07113 Mouse
    • Unigene: 487062 Human
    • Unigene: 673278 Human
    • Unigene: 26553 Mouse
    • Unigene: 486511 Mouse
    see all
  • 别名

    • 300 kDa mannose 6 phosphate receptor antibody
    • 300 kDa mannose 6-phosphate receptor antibody
    • Cation independent mannose 6 phosphate receptor antibody
    • Cation-independent mannose-6-phosphate receptor antibody
    • CD222 antibody
    • CD222 antigen antibody
    • CI Man 6 P receptor antibody
    • CI Man-6-P receptor antibody
    • CI MPR antibody
    • CI-M6PR antibody
    • CI-MPR antibody
    • CIMPR antibody
    • IGF 2 receptor antibody
    • IGF 2R antibody
    • IGF II receptor antibody
    • IGF-II receptor antibody
    • IGF2 receptor antibody
    • Igf2r antibody
    • Insulin like growth factor 2 receptor antibody
    • Insulin like growth factor II receptor antibody
    • Insulin-like growth factor 2 receptor antibody
    • Insulin-like growth factor II receptor antibody
    • M6P R antibody
    • M6P/IGF2 receptor antibody
    • M6P/IGF2R antibody
    • M6PR antibody
    • mannose 6 phosphate receptor antibody
    • mannose 6 phosphate receptor, cation independent antibody
    • MPR 300 antibody
    • MPR300 antibody
    • MPRI antibody
    • MPRI_HUMAN antibody
    see all

图片

  • Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: M6PR (cation independent) knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: A549 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab124767 observed at 274 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab124767 was shown to specifically react with M6PR (cation independent) in wild-type HAP1 cells as signal was lost in M6PR (cation independent) knockout cells. Wild-type and M6PR (cation independent) knockout samples were subjected to SDS-PAGE. Ab124767 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/50000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)

    ab124767 staining M6PR in wild-type HAP1 cells (top panel) and IGF2R knockout HAP1 cells (bottom panel). The cells were fixed with 100% MeOH for 5 min. , permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab124767 at 1μg/ml and ab195889 (Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 594)) at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.









    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    All lanes : Anti-M6PR (cation independent) antibody [EPR6599] (ab124767) at 1/50000 dilution (purified)

    Lane 1 : C6 (rat glioma) whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 3 : NIH/3T3 (mouse embryo) whole cell lysate
    Lane 4 : Mouse heart tissue lysate
    Lane 5 : Mouse kidney tissue lysate
    Lane 6 : Mouse spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 274 kDa
    Observed band size: 300 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer 5% NFDM/TBST
  • Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    All lanes : Anti-M6PR (cation independent) antibody [EPR6599] (ab124767) at 1/50000 dilution (unpurified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Caco-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 274 kDa

  • Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    Western blot - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    All lanes : Anti-M6PR (cation independent) antibody [EPR6599] (ab124767) at 1/200000 dilution (purified)

    Lane 1 : Jurkat (human acute T cell leukemia) whole cell lysate
    Lane 2 : HEK293 (human embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 274 kDa
    Observed band size: 300 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer 5% NFDM/TBST
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)

    Immunohistochemical staining of paraffin embedded rat colon tissue section labelling M6PR with purified ab124767 at dilution of 1/500. The secondary antibody used was ab97051 Goat Anti-Rabbit IgG H&L (HRP), at a dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)

    Immunohistochemical staining of paraffin embedded mouse colon tissue section labelling M6PR with purified ab124767 at dilution of 1/500. The secondary antibody used was ab97051 Goat Anti-Rabbit IgG H&L (HRP), at a dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)

    Immunohistochemical staining of paraffin embedded human thyroid carcinoma tissue section labelling M6PR with purified ab124767 at dilution of 1/500. The secondary antibody used was ab97051 Goat Anti-Rabbit IgG H&L (HRP), at a dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)

    Immunohistochemical analysis of formalin fixed, paraffin embedded Human papillary carcinoma tissue section labelling Mannose 6 Phosphate Receptor (Cation independent) with unpurified ab124767 at dilution of 1/250.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)

    Immunohistochemical analysis of formalin fixed, paraffin embedded Human tonsil tissue section labelling Mannose 6 Phosphate Receptor (Cation independent) with unpurified ab124767 at dilution of 1/250.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)

    Immunocytochemistry/Immunofluorescence analysis of Jurkat (human acute T cell leukemia) cells labelling M6PR with purified ab124767 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/1000) using ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary. Nuclei couterstained with DAPI (blue).

    For negative control 1, rabbit primary antibody was used, followed by anti-mouse secondary antibody (ab150120). For negative control 2, mouse primary antibody (ab7291) was used followed by anti-rabbit secondary antibody (ab150077).  

  • Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)

    Immunocytochemistry/immunofluorescence analysis of 293T cells labelling Mannose 6 Phosphate Receptor (Cation independent) with unpurified ab124767 at dilution of 1/100.

  • Flow Cytometry - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    Flow Cytometry - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)

    Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labelling M6PR with purified ab124767 at 1/150 (red). Cells were fixed with 4% paraformaldehyde. Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • OI-RD Scanning - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    OI-RD Scanning - Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)
    Anti-M6PR (cation independent) antibody [EPR6599] (ab124767)

实验方案

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (39)

发表研究结果有使用 ab124767?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab124767 被引用在 39 文献中.

  • Curtis ME  et al. Dysregulation of the Retromer Complex System in Down Syndrome. Ann Neurol 88:137-147 (2020). PubMed: 32320094
  • Han J  et al. Involvement of CASP9 (caspase 9) in IGF2R/CI-MPR endosomal transport. Autophagy N/A:1-17 (2020). PubMed: 32397873
  • Cui L  et al. The Lysosomal Membrane Protein Lamp2 Alleviates Lysosomal Cell Death by Promoting Autophagic Flux in Ischemic Cardiomyocytes. Front Cell Dev Biol 8:31 (2020). PubMed: 32117965
  • Boivin M  et al. Reduced autophagy upon C9ORF72 loss synergizes with dipeptide repeat protein toxicity in G4C2 repeat expansion disorders. EMBO J 39:e100574 (2020). PubMed: 31930538
  • Li JG  et al. A pharmacological chaperone improves memory by reducing Aß and tau neuropathology in a mouse model with plaques and tangles. Mol Neurodegener 15:1 (2020). PubMed: 31964406
View all Publications for this product

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Western blot
Sample
Human Cell lysate - whole cell (HEK293FT cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
HEK293FT cells
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
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Abcam user community

Verified customer

提交于 Nov 10 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-M6PR (cation independent) antibody [EPR6599]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (INS1)
Permeabilization
Yes - Tx100
Specification
INS1
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

提交于 May 21 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-M6PR (cation independent) antibody [EPR6599]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK 293T cells)
Permeabilization
Yes - 0.1% Saponin, 20% FCS in PBS
Specification
HEK 293T cells
Blocking step
0.1% Saponin, 20% FCS in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 21°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

提交于 Jun 18 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - Triton X100
Specification
HeLa
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Jun 07 2019

Western blot abreview for Anti-M6PR (cation independent) antibody [EPR6599]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (293T)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
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Abcam user community

Verified customer

提交于 Jun 07 2019

Question

Dear All,
a customer of ours is interested in your: Anti-Mannose 6 Phosphate Receptor (Cation independent) antibody [EPR6599] (ab124767) - 100Ul
and she would like to ask if he can proceed by diluting the mother antibody. In case of positive response I would ask if the dilutions should be made in 49% PBS, 0.01% Sodium Azide, 50% Glycerol, 0.05% BSA

Thanks and kind regards

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Abcam community

Verified customer

Asked on Feb 04 2013

Answer

Thank you for your enquiry and interest in our products.

We would recommend diluting this antibody for the tested applications. The storage buffer of this antibody contains PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% so PBS can be used as diluent. Please advise your customer to check the recommended dilution range for the guaranteed applications.

If you need any further assistance in the future, please do not hesitate to contact me.

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Abcam Scientific Support

回复于 Feb 04 2013

Western blot abreview for Anti-Mannose 6 Phosphate Receptor (Cation independent) antibody [EPR6599]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Hek293)
Loading amount
30 µg
Specification
Hek293
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Jan 29 2013

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