Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Wound)
Permeabilization
No
Specification
Wound
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking solution
Dilution
1/100
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Cy™3 AffiniPure Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
Control staining with Rabbit IgG, polyclonal - Isotype Control (ab37415) was performed which led to a comparable staining
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested in IHC-Fr and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 May 06 2016