Anti-Lysozyme抗体(ab391)

Reviews (1)Q&A (5)References (4)

概述

  • 产品名称
    Anti-Lysozyme抗体
    参阅全部 Lysozyme 一抗
  • 描述
    兔多克隆抗体to Lysozyme
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IP, ELISA, ICCmore details
  • 种属反应性
    与反应: Chicken
  • 免疫原

    Lysozyme from Hen Egg White

  • 阳性对照
    • Assayed against 1.0 ug of Lysozyme from hen egg white in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 0.424% Potassium phosphate
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lysozyme.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000.
IP 1/100.
ELISA 1/25000.
ICC 1/500 - 1/2000.

靶标

  • 功能
    Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
  • 疾病相关
    Amyloidosis 8
  • 序列相似性
    Belongs to the glycosyl hydrolase 22 family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
    • 别名
      • 1 4 beta N acetylmuramidase C antibody
      • 1 antibody
      • 4-beta-N-acetylmuramidase C antibody
      • EC 3.2.1.17 antibody
      • LYSC_HUMAN antibody
      • Lysosyme antibody
      • Lysozyme (renal amyloidosis) antibody
      • Lysozyme C antibody
      • Lysozyme C precursor antibody
      • LYZ antibody
      • LZM antibody
      • Renal amyloidosis antibody
      see all

    图片

    • Western blot - Anti-Lysozyme antibody (ab391)
      Western blot - Anti-Lysozyme antibody (ab391)
      Anti-Lysozyme antibody (ab391) at 1/1000 dilution + Lysozyme (Hen Egg White) at 0.05 µg

      Secondary
      Rabbit HRP conjugate at 1/40000 dilution

    文献

    This product has been referenced in:
    • Bao Y  et al. O-specific polysaccharide confers lysozyme resistance to extraintestinal pathogenic Escherichia coli. Virulence 9:666-680 (2018). Read more (PubMed: 29405825) »
    • He T  et al. Proteomic comparison by iTRAQ combined with mass spectrometry of egg white proteins in laying hens (Gallus gallus) fed with soybean meal and cottonseed meal. PLoS One 12:e0182886 (2017). Read more (PubMed: 28813468) »
    See all 4 Publications for this product

    发表研究结果有使用 ab391？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    1-6 of 6 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Lysozyme antibody

     Inconclusive
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Sample
    Chicken Tissue sections (embryo)
    Specification
    embryo
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Anna Keyle

    Verified customer

    提交于 Jun 12 2014

    Question

    I have a customer looking for an alkaline phosphatase conjugated lysozyme antibody, and they specifically asked about AB391. I don't see any alk phos conjugates listed on your web, but they have also asked if you can custom conjugate this antibody if a conjugate is not available. Can you please let me know asap? Thanks in advance, Jessica

    Read More
    Answer

    Thank you for your enquiry. We currently do not have a lysozyme antibody conjugated to AP and we are unable to custom conjugate antibodies.

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    Question

    I would like to perform immunohistochemistry using ab391 on formalin-fixed, paraffin-embedded mouse intestine sections. Do you think your antibody will work for this?

    Read More
    Answer

    This antibody has not yet been tested for use with IHC. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher. If you have more questions, please contact us again.

    Read More

    Question

    Dear Sir or Madam, I would like to dectect small amounts of lysozyme by immunoprecipitation and western blot from cell extracts. Have you tested your antibody in these conditions? Thanks in advance Dr. Manuel Muniz

    Read More
    Answer

    Yes, this antibody has been tested by immunoprecipitation and Western blot. The suggested working dilution is 1/4000 to 1/10000, although optimisation in your own lab is recommended.

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    Question

    I wanted to ask about the anti-Lysozyme (product code:ab391, batch 5732) which I recently purchased from you. It appears by Western blotting that although the antibody reacts strongly with egg white lysozyme (detects ca. 0.1ng), there is also reaction with many E. coli proteins. I wanted to ask whether this result could be due to bacterial material in the adjuvant used in immunization? Or is this result unexpected or incorrect? I am using this antibody in conjunction with cloning in E. coli, so that the cross-reaction is a definite problem. Thank you for any information or assistance you can provide.

    Read More
    Answer

    Polyclonal antibodies are normally produced in SPF (specific pathogen free) rabbits and these rabbits are free of infection with E. coli. Even with these precautions, quite a lot of polyclonal antibodies give cross-reaction with E. coli proteins. Try using less of the antibody and/or shorter incubation times to reduce the background. If this doesn't help, a good method is to adsorb the antibody with a E. coli protein preparation that does not contain your antigen of interest.

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    Question

    hello sir; can I use this product for dog antigens in immunoblotting? thank you

    Read More
    Answer

    This antibody is known to work in immunoblotting, however it's reactivity against dog lysozyme has not been determined. As the antibody was raised against lysozyme from chicken egg white, it is uncertain whether it will react with canine.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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