Application
Western blot
Sample
Human Tissue lysate - whole (human gut mucosa)
Loading amount
30 µg
Specification
human gut mucosa
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk TBST
Secondary antibody
Name
Non-Abcam antibody was used: DAKO
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
Pierce ECL
Exposure
10 second(s)
Bands
Specific: 15 kDa
Positive control
these samples
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 03 2013