Anti-Lysozyme抗体(ab2408)
Key features and details
- Rabbit polyclonal to Lysozyme
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Human
- Isotype: unknown
概述
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产品名称
Anti-Lysozyme抗体
参阅全部 Lysozyme 一抗 -
描述
兔多克隆抗体to Lysozyme -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IHC-Frmore details -
种属反应性
与反应: Human -
免疫原
Lysozyme isolated from urine of patients with monocytic leukemia.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
纯度
Proprietary Purification -
克隆
多克隆 -
同种型
unknown -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab2408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/50 - 1/100. | |
| IHC-Fr | Use at an assay dependent concentration. |
靶标
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功能
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. -
疾病相关
Amyloidosis 8 -
序列相似性
Belongs to the glycosyl hydrolase 22 family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4069 Human
- Omim: 153450 Human
- SwissProt: P61626 Human
- Unigene: 524579 Human
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别名
- 1 4 beta N acetylmuramidase C antibody
- 1 antibody
- 4-beta-N-acetylmuramidase C antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody (ab2408)Ab2408 staining lysozyme in human colon. Staining is localised to cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody (ab2408)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining Lysozyme with ab2408.
文献 (3)
ab2408 被引用在 3 文献中.
- Mao Y et al. Processed human amniotic fluid retains its antibacterial activity. J Transl Med 17:68 (2019). PubMed: 30823930
- Burg JM et al. Lysine-Specific Demethylase 1A (KDM1A/LSD1): Product Recognition and Kinetic Analysis of Full-Length Histones. Biochemistry 55:1652-62 (2016). PubMed: 26673564
- Forbester JL et al. Interaction of Salmonella enterica Serovar Typhimurium with Intestinal Organoids Derived from Human Induced Pluripotent Stem Cells. Infect Immun 83:2926-34 (2015). IHC . PubMed: 25964470
