Key features and details
- Mouse monoclonal [LYN-01] to Lyn
- Suitable for: Flow Cyt, ICC/IF, IP, WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
参阅全部 Lyn 一抗
描述小鼠单克隆抗体[LYN-01] to Lyn
经测试应用适用于: Flow Cyt, ICC/IF, IP, WB, ICCmore details
种属反应性与反应: Mouse, Rat, Human
Recombinant fragment corresponding to Human Lyn aa 8-238.
GKDSLSDDGVDLK TQPVRNTERT IYVRDPTSNK QQRPVPESQL LPGQRFQTKDPEEQGDIVVA LYPYDGIHPD DLSFKKGEKM KVLEEHGEWW KAKSLLTKKE GFIPSNYVAKLNTLETEEWF FKDITRKDAE RQLLAPGNSA GAFLIRESET LKGSFSLSVR DFDPVHGDVIKHYKIRSLDN GGYYISPRIT FPCISDMIKH YQKQADGLCR RLEKACISPK PQKPWDKD
Lyn is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. Like all members of the src family, lyn is thought to participate in signal transduction from cell surface receptors that lack intrinsic tyrosine kinase activity. It is associated with a number of cell surface receptors including the B cell antigen receptor and immunoglobulin E receptor (FceRI).
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.097% Sodium azide
Concentration information loading...
纯度Protein A purified
纯化说明Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.
Primary antibody说明Lyn is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. Like all members of the src family, lyn is thought to participate in signal transduction from cell surface receptors that lack intrinsic tyrosine kinase activity. It is associated with a number of cell surface receptors including the B cell antigen receptor and immunoglobulin E receptor (FceRI).
Our Abpromise guarantee covers the use of ab1890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.|
|ICC||Use at an assay dependent concentration.|
功能Down regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents.
组织特异性Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.
序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
结构域The protein kinase domain plays an important role in its localization in the cell membrane.
翻译后修饰Ubiquitinated. Ubiquitination is SH3-dependent.
细胞定位Cell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport.
- Information by UniProt
形式This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and SRC (P12931). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
- AA407514 antibody
- EC 18.104.22.168 antibody
- FLJ26625 antibody
Anti-Lyn antibody [LYN-01] (ab1890) at 1/1000 dilution +
Recombinant human Lyn protein (ab60306) at 0.01 µg
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Exposure time: 4 minutes
Anti-Lyn antibody [LYN-01] (ab1890) at 1/1000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg
HRP-conjugated anti-mouse at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
Central Panel: ab1890 staining Lyn in human PMN cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% TritonX-100 + 2% BSA in PBS and blocked with 2% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody 1/100 in blocking buffer for 4 hours at 37°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG, dilution 1/100, was used as secondary antibody.
Left-hand panel: Nuclei counterstained with DAPI (blue).
Right-hand panel: Overlay
Overlay histogram showing Ramos cells stained with ab1890 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1890, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab1890 被引用在 16 文献中.
- Brian BF et al. Unique-region phosphorylation targets LynA for rapid degradation, tuning its expression and signaling in myeloid cells. Elife 8:N/A (2019). PubMed: 31282857
- Gavali S et al. Estrogen enhances human osteoblast survival and function via promotion of autophagy. Biochim Biophys Acta Mol Cell Res 1866:1498-1507 (2019). PubMed: 31255720
- Lin J et al. Characterizing the molecular mechanisms of acquired temozolomide resistance in the U251 glioblastoma cell line by protein microarray. Oncol Rep 39:2333-2341 (2018). PubMed: 29565460
- Tornillo G et al. Dual Mechanisms of LYN Kinase Dysregulation Drive Aggressive Behavior in Breast Cancer Cells. Cell Rep 25:3674-3692.e10 (2018). PubMed: 30590041
- Gavali S et al. LYN, a key mediator in estrogen-dependent suppression of osteoclast differentiation, survival, and function. Biochim Biophys Acta Mol Basis Dis 1865:547-557 (2018). PubMed: 30579930