Application
Western blot
Sample
Chinese Hamster Cell lysate - whole cell (CHO cell lysate)
Loading amount
50000 cells
Specification
CHO cell lysate
Gel Running Conditions
Reduced Denaturing
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: Pierce TBS Super Block
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/1000
Detection
Detection method
Super Signa West Dura
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 21 2008