Anti-LXR alpha抗体(ab3585)
Key features and details
- Rabbit polyclonal to LXR alpha
- Suitable for: ICC, WB, IHC-FoFr, ICC/IF
- Reacts with: Mouse, Rat, Human, Chinese hamster
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-LXR alpha抗体
参阅全部 LXR alpha 一抗 -
描述
兔多克隆抗体to LXR alpha -
宿主
Rabbit -
特异性
Detects recombinant Human LXR alpha. This antibody does not detect recombinant Human LXR beta. -
经测试应用
适用于: ICC, WB, IHC-FoFr, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human, Chinese hamster
预测可用于: Cow
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免疫原
Synthetic peptide corresponding to Human LXR alpha aa 318-329.
Sequence:DFSYNREDFAKA
(Peptide available asab5021) -
阳性对照
- WB: MCF7, THP-1, A549, SH-SY5Y, HL-60, KARPAS 299 cells. ICC: MCF7, HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Liver X Receptors (LXRs) are oxysterol activated nuclear receptors which are involved in the regulation of genesis and metabolism of cholesterols and bile acids. LXRs form heterodimeric complexes with the retinoic acid receptors (RARs) once activated by their respective oxysterol ligands. Two known isoforms, alpha and beta, are differentially expressed; the alpha isoform predominantly expressed in liver, whereas beta isoform expression has been shown to be ubiquitous. -
克隆
多克隆 -
同种型
IgG -
研究领域
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
1/50 - 1/500.
|
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| EMSA |
Use at an assay dependent concentration.
|
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| Gel Shift Assay |
Use at an assay dependent concentration.
|
|
| WB | (1) |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 50 kDa.
|
| IHC-FoFr |
Use at an assay dependent concentration. PubMed: 22836489
|
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| ICC/IF | (2) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
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ICC
1/50 - 1/500. |
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EMSA
Use at an assay dependent concentration. |
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Gel Shift Assay
Use at an assay dependent concentration. |
|
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 50 kDa. |
|
IHC-FoFr
Use at an assay dependent concentration. PubMed: 22836489 |
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ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. -
组织特异性
Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals. -
序列相似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 507176 Cow
- Entrez Gene: 10062 Human
- Entrez Gene: 22259 Mouse
- Entrez Gene: 58852 Rat
- Omim: 602423 Human
- SwissProt: Q5E9B6 Cow
- SwissProt: Q13133 Human
- SwissProt: Q9Z0Y9 Mouse
see all -
别名
- Liver X receptor alpha antibody
- LXR a antibody
- LXRA antibody
see all
图片
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Western blot - Anti-LXR alpha antibody (ab3585)All lanes : Anti-LXR alpha antibody (ab3585) at 2 µg/ml
Lane 1 : MCF7
Lane 2 : THP-1
Lane 3 : A549
Lane 4 : SH-SY5Y
Lane 5 : HL-60
Lane 6 : KARPAS 299
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/4000 dilution
Predicted band size: 50 kDaWestern blot analysis was performed on nuclear enriched extracts.
A 50 kDa band corresponding to LXR alpha was observed across the cell lines tested.
Blocking buffer: 5 % skimmed milk
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Immunocytochemistry - Anti-LXR alpha antibody (ab3585)Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.
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Immunocytochemistry - Anti-LXR alpha antibody (ab3585)Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.
实验方案
数据表及文件
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Datasheet download
文献 (19)
ab3585 被引用在 19 文献中.
- Li T et al. The marine-derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRa and PPARa. J Cell Mol Med 24:3384-3398 (2020). PubMed: 31981312
- Yu W et al. Activation of LXRß Signaling in the Amygdala Confers Anxiolytic Effects Through Rebalancing Excitatory and Inhibitory Neurotransmission upon Acute Stress. Neurotherapeutics 17:1253-1270 (2020). PubMed: 32297184
- Xu D et al. SIRT2 plays a novel role on progesterone, estradiol and testosterone synthesis via PPARs/LXRa pathways in bovine ovarian granular cells. J Steroid Biochem Mol Biol 185:27-38 (2019). PubMed: 30009951
- Yin J et al. The fucoidan from the brown seaweed Ascophyllum nodosum ameliorates atherosclerosis in apolipoprotein E-deficient mice. Food Funct 10:5124-5139 (2019). PubMed: 31364648
- Jain J et al. Establishment and Comparison of Pathogenicity and Related Neurotropism in Two Age Groups of Immune Competent Mice, C57BL/6J Using an Indian Isolate of Chikungunya Virus (CHIKV). Viruses 11:N/A (2019). PubMed: 31242674
