Anti-LXR alpha抗体(ab3585)
Key features and details
- Rabbit polyclonal to LXR alpha
- Suitable for: ICC
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-LXR alpha抗体
参阅全部 LXR alpha 一抗 -
描述
兔多克隆抗体to LXR alpha -
宿主
Rabbit -
特异性
Detects recombinant Human LXR alpha. This antibody does not detect recombinant Human LXR beta. -
经测试应用
适用于: ICCmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow
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免疫原
Synthetic peptide corresponding to Human LXR alpha aa 318-329.
Sequence:DFSYNREDFAKA
(Peptide available asab5021) -
常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Liver X Receptors (LXRs) are oxysterol activated nuclear receptors which are involved in the regulation of genesis and metabolism of cholesterols and bile acids. LXRs form heterodimeric complexes with the retinoic acid receptors (RARs) once activated by their respective oxysterol ligands. Two known isoforms, alpha and beta, are differentially expressed; the alpha isoform predominantly expressed in liver, whereas beta isoform expression has been shown to be ubiquitous. -
克隆
多克隆 -
同种型
IgG -
研究领域
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab3585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | Use at an assay dependent concentration. |
靶标
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功能
Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. -
组织特异性
Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals. -
序列相似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 507176 Cow
- Entrez Gene: 10062 Human
- Entrez Gene: 22259 Mouse
- Omim: 602423 Human
- SwissProt: Q5E9B6 Cow
- SwissProt: Q13133 Human
- SwissProt: Q9Z0Y9 Mouse
- Unigene: 438863 Human
see all -
别名
- Liver X receptor alpha antibody
- LXR a antibody
- LXRA antibody
see all
图片
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Immunocytochemistry - Anti-LXR alpha antibody (ab3585)
Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.
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Immunocytochemistry - Anti-LXR alpha antibody (ab3585)Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.
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Immunocytochemistry - Anti-LXR alpha antibody (ab3585)This image is courtesy of an Abreview submitted by Dr Mahesh Mathrubuthamab3585 at 1/100 staining hamster CHO-K1 cells expressing human LXRa by ICC/IF. The cells were formaldehyde fixed, permeabilized and blocked before being stained with the antibody for 1 hour at 22°C. A FITC labelled goat anti-rabbit antibody was used as the secondary.
实验方案
数据表及文件
文献 (18)
ab3585 被引用在 18 文献中.
- Li T et al. The marine-derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRa and PPARa. J Cell Mol Med 24:3384-3398 (2020). PubMed: 31981312
- Xu D et al. SIRT2 plays a novel role on progesterone, estradiol and testosterone synthesis via PPARs/LXRa pathways in bovine ovarian granular cells. J Steroid Biochem Mol Biol 185:27-38 (2019). PubMed: 30009951
- Yin J et al. The fucoidan from the brown seaweed Ascophyllum nodosum ameliorates atherosclerosis in apolipoprotein E-deficient mice. Food Funct 10:5124-5139 (2019). PubMed: 31364648
- Jain J et al. Establishment and Comparison of Pathogenicity and Related Neurotropism in Two Age Groups of Immune Competent Mice, C57BL/6J Using an Indian Isolate of Chikungunya Virus (CHIKV). Viruses 11:N/A (2019). PubMed: 31242674
- Cheng Y et al. Downregulation of microRNA-1 attenuates glucose-induced apoptosis by regulating the liver X receptor a in cardiomyocytes. Exp Ther Med 16:1814-1824 (2018). PubMed: 30186406
