重组Anti-Lumican抗体[EPR8898(2)] - BSA and Azide free (ab198974)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8898(2)] to Lumican - BSA and Azide free
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Lumican抗体[EPR8898(2)] - BSA and Azide free
参阅全部 Lumican 一抗 -
描述
兔单克隆抗体[EPR8898(2)] to Lumican - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IF, Flow Cyt (Intra)more details
不适用于: IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human Lumican. The exact sequence is proprietary.
Database link: P51884 -
阳性对照
- Saos-2, fetal skin, fetal heart, Jurkat, Y79 and 293T lysates , Human kidney and Human skin tissues, Saos-2 and 293T cells
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常规说明
ab198974 is the carrier-free version of ab168348.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: 100% PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8898(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab198974于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).
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ICC/IF | (2) |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa). |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody. |
靶标
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组织特异性
Cornea and other tissues. -
序列相似性
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
发展阶段
Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan. -
翻译后修饰
Sulfated on tyrosine residue(s). -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 4060 Human
- Entrez Gene: 17022 Mouse
- Entrez Gene: 81682 Rat
- Omim: 600616 Human
- SwissProt: P51884 Human
- SwissProt: P51885 Mouse
- SwissProt: P51886 Rat
- Unigene: 406475 Human
see all -
别名
- Keratan sulfate proteoglycan lumican antibody
- KSPG lumican antibody
- LDC antibody
see all
图片
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Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Lumican with ab168348 at 1/50.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168348).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular flow cytometrical analysis of permeabilized 293T cells with ab168348 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168348).
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Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Lumican with ab168348 at 1/50.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168348).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (2)
ab198974 被引用在 2 文献中.
- Rebbeck CA et al. Gene expression signatures of individual ductal carcinoma in situ lesions identify processes and biomarkers associated with progression towards invasive ductal carcinoma. Nat Commun 13:3399 (2022). PubMed: 35697697
- Zheng Z et al. Fibromodulin-deficiency alters temporospatial expression patterns of transforming growth factor-ß ligands and receptors during adult mouse skin wound healing. PLoS One 9:e90817 (2014). IHC-P ; Mouse . PubMed: 24603701