重组Anti-Lumican抗体[EPR8898(2)] (ab168348)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8898(2)] to Lumican
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Lumican抗体[EPR8898(2)]
参阅全部 Lumican 一抗 -
描述
兔单克隆抗体[EPR8898(2)] to Lumican -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human Lumican aa 250-350. The exact sequence is proprietary.
Database link: P51884 -
阳性对照
- WB: Saos-2, fetal skin, fetal heart, Jurkat, Y79 and HEK-293T lysates. IHC-P: Human kidney and Human skin tissues. ICC/IF: Saos-2 cells. Flow Cyt (intra): HEK-293T cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8898(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab168348于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).
|
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IHC-P | (6) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
ICC/IF | (3) |
1/250 - 1/500.
|
说明 |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
靶标
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组织特异性
Cornea and other tissues. -
序列相似性
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
发展阶段
Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan. -
翻译后修饰
Sulfated on tyrosine residue(s). -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 4060 Human
- Entrez Gene: 17022 Mouse
- Entrez Gene: 81682 Rat
- Omim: 600616 Human
- SwissProt: P51884 Human
- SwissProt: P51885 Mouse
- SwissProt: P51886 Rat
- Unigene: 406475 Human
see all -
别名
- Keratan sulfate proteoglycan lumican antibody
- KSPG lumican antibody
- LDC antibody
see all
图片
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All lanes : Anti-Lumican antibody [EPR8898(2)] (ab168348) at 1/1000 dilution
Lane 1 : Saos-2 lysate
Lane 2 : Fetal skin lysate
Lane 3 : Fetal heart lysate
Lane 4 : Jurkat lysate
Lane 5 : Y79 lysate
Lane 6 : 293T lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Lumican with ab168348 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of Saos-2 cells labeling Lumican with ab168348 at 1/250.
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Intracellular flow cytometrical analysis of permeabilized 293T cells with ab168348 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
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Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Lumican with ab168348 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (30)
ab168348 被引用在 30 文献中.
- Chow SE et al. Nuclear p120 catenin is a component of the perichromosomal layer and coordinates sister chromatid segregation during mitosis in lung cancer cells. Cell Death Dis 13:526 (2022). PubMed: 35660718
- Wang K et al. Lumican is elevated in the lung in human and experimental acute respiratory distress syndrome and promotes early fibrotic responses to lung injury. J Transl Med 20:392 (2022). PubMed: 36059026
- Wu J et al. Effects of lumican expression on the apoptosis of scleral fibroblasts: In vivo and in vitro experiments. Exp Ther Med 21:495 (2021). PubMed: 33791004
- Kafarnik C et al. Canine Corneal Stromal Cells Have Multipotent Mesenchymal Stromal Cell Properties In Vitro. Stem Cells Dev 29:425-439 (2020). PubMed: 31973649
- Denes BJ et al. Core Matrisome Protein Signature During Periodontal Ligament Maturation From Pre-occlusal Eruption to Occlusal Function. Front Physiol 11:174 (2020). PubMed: 32194440