重组Anti-LSP1抗体[EPR5997] (ab133506)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5997] to LSP1
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-LSP1抗体[EPR5997]
参阅全部 LSP1 一抗 -
描述
兔单克隆抗体[EPR5997] to LSP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
不适用于: IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human LSP1 aa 300-400 (C terminal). The exact sequence is proprietary.
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阳性对照
- WB: Daudi, Ramos and HuT78 cell lysates. Mouse spleen and Rat brain tissue lysates.IHC-P: Human colon, Mouse spleen, Rat spleen and Human tonsil tissue. ICC/IF: Daudi Flow Cyt (intra): Daudi
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.02 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5997 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133506于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 37 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100.
For unpurified use at 1/500 - 1/1000. |
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Flow Cyt (Intra) |
1/100.
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 37 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100. For unpurified use at 1/500 - 1/1000. |
Flow Cyt (Intra)
1/100. |
靶标
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功能
May play a role in mediating neutrophil activation and chemotaxis. -
组织特异性
Activated T-lymphocytes. -
翻译后修饰
Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4046 Human
- Entrez Gene: 16985 Mouse
- Entrez Gene: 361680 Rat
- Omim: 153432 Human
- SwissProt: P33241 Human
- SwissProt: P19973 Mouse
- Unigene: 56729 Human
- Unigene: 234003 Mouse
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别名
- 47 kDa actin binding protein antibody
- 47 kDa actin-binding protein antibody
- 52 kDa phosphoprotein antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Anti-LSP1 antibody [EPR5997] (ab133506) at 1/10000 dilution (Purified) + Rat brain lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa -
All lanes : Anti-LSP1 antibody [EPR5997] (ab133506) at 1/10000 dilution (Purified)
Lane 1 : Mouse spleen lysates
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 3 : HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunocytochemistry/ Immunofluorescence analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling LSP1 with purified ab133506 at 1:100 dilution (9.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling LSP1 with purified ab133506 at 1/100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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All lanes : Anti-LSP1 antibody [EPR5997] (ab133506) at 1/10000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : HuT78 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 37 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling LSP1 with ab133506 at 1/250 dilution.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling LSP1 with ab133506 at 1/250 dilution.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab133506 被引用在 2 文献中.
- Chen X et al. Comprehensive Gene Expression Analysis in NMIBC Using RNA-seq Reveals New Therapy Strategies. Front Oncol 9:523 (2019). PubMed: 31293967
- Zhang H et al. Lymphocyte-specific protein 1 inhibits the growth of hepatocellular carcinoma by suppressing ERK1/2 phosphorylation. FEBS Open Bio 6:1227-1237 (2016). PubMed: 28255535