产品名称Anti-LRRK2 (phospho T2483)抗体[MJF-R8 (21-2e)]
参阅全部 LRRK2 一抗
描述兔单克隆抗体[MJF-R8 (21-2e)] to LRRK2 (phospho T2483)
经测试应用适用于: WBmore details
Synthetic phosphopeptide corresponding to residues surrounding Threonine 2483 of Human LRRK2 (UniProt ID: Q5S007).
- HEK293 cells transiently transfected with wild-type or mutant LRRK2.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
纯度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab156577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 286 kDa.|
功能Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays a role in synaptic vesicle trafficking. Phosphorylates PRDX3. Has GTPase activity. May play a role in the phosphorylation of proteins central to Parkinson disease.
组织特异性Expressed in the brain. Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.
疾病相关Parkinson disease 8
序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 12 LRR (leucine-rich) repeats.
Contains 1 protein kinase domain.
Contains 1 Roc domain.
Contains 7 WD repeats.
结构域The seven-bladed WD repeat region is critical for synaptic vesicle trafficking and mediates interaction with multiple vesicle-associated presynaptic proteins.
The Roc domain mediates homodimerization and regulates kinase activity.
细胞定位Membrane. Cytoplasm. Perikaryon. Mitochondrion. Golgi apparatus. Cell projection, axon. Cell projection, dendrite. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Endosome. Lysosome. Mitochondrion outer membrane. Mitochondrion inner membrane. Mitochondrion matrix. Predominantly associated with intracytoplasmic vesicular and membranous structures (By similarity). Localized in the cytoplasm and associated with cellular membrane structures. Predominantly associated with the mitochondrial outer membrane of the mitochondria. Colocalized with RAB29 along tubular structures emerging from Golgi apparatus. Localizes in intracytoplasmic punctate structures of neuronal perikarya and dendritic and axonal processes.
- Information by UniProt
- augmented in rheumatoid arthritis 17 antibody
- AURA17 antibody
- Dardarin antibody
All lanes : Anti-LRRK2 (phospho T2483) antibody [MJF-R8 (21-2e)] (ab156577) at 1/1000 dilution
Lane 1 : HEK293 cells transiently transfected with wild type LRRK2
Lane 2 : HEK293 cells transiently transfected with T2483
Lane 3 : HEK293 cells transiently transfected with G2019S
Lane 4 : HEK293 cells transiently transfected with Kinase dead
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 286 kDa
LRRK2 protein was immunoprecipitated and subjected to an in vitro kinase assay in the presence (+) or absence (-) of ATP.
This product has been referenced in:
- Schmidt SH et al. The dynamic switch mechanism that leads to activation of LRRK2 is embedded in the DFG? motif in the kinase domain. Proc Natl Acad Sci U S A 116:14979-14988 (2019). Read more (PubMed: 31292254) »
- Muda K et al. Parkinson-related LRRK2 mutation R1441C/G/H impairs PKA phosphorylation of LRRK2 and disrupts its interaction with 14-3-3. Proc Natl Acad Sci U S A 111:E34-43 (2014). WB . Read more (PubMed: 24351927) »