概述

  • 产品名称

    Anti-LRRK2 (phospho S1292)抗体[MJFR-19-7-8]
    参阅全部 LRRK2 一抗
  • 描述

    兔单克隆抗体[MJFR-19-7-8] to LRRK2 (phospho S1292)
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human LRRK2 aa 1250-1350 (phospho S1292). The exact sequence is proprietary.
    Database link: Q5S007

  • 阳性对照

    • GFP-LRRK2 wt transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose; GFP-LRRK2 mutant G 2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose; LRRK2 wt transfected HEK293 lysate; LRRK2 mutant G2019S transfected HEK293 lysate.
  • 常规说明

    This antibody was developed with support from The Michael J. Fox Foundation.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    MJFR-19-7-8
  • 同种型

    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab203181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 286 kDa (predicted molecular weight: 286 kDa).

靶标

  • 功能

    Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays a role in synaptic vesicle trafficking. Phosphorylates PRDX3. Has GTPase activity. May play a role in the phosphorylation of proteins central to Parkinson disease.
  • 组织特异性

    Expressed in the brain. Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.
  • 疾病相关

    Parkinson disease 8
  • 序列相似性

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 12 LRR (leucine-rich) repeats.
    Contains 1 protein kinase domain.
    Contains 1 Roc domain.
    Contains 7 WD repeats.
  • 结构域

    The seven-bladed WD repeat region is critical for synaptic vesicle trafficking and mediates interaction with multiple vesicle-associated presynaptic proteins.
    The Roc domain mediates homodimerization and regulates kinase activity.
  • 翻译后修饰

    Autophosphorylated.
  • 细胞定位

    Membrane. Cytoplasm. Perikaryon. Mitochondrion. Golgi apparatus. Cell projection, axon. Cell projection, dendrite. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Endosome. Lysosome. Mitochondrion outer membrane. Mitochondrion inner membrane. Mitochondrion matrix. Predominantly associated with intracytoplasmic vesicular and membranous structures (By similarity). Localized in the cytoplasm and associated with cellular membrane structures. Predominantly associated with the mitochondrial outer membrane of the mitochondria. Colocalized with RAB29 along tubular structures emerging from Golgi apparatus. Localizes in intracytoplasmic punctate structures of neuronal perikarya and dendritic and axonal processes.
  • Information by UniProt
  • 数据库链接

  • 别名

    • augmented in rheumatoid arthritis 17 antibody
    • AURA17 antibody
    • Dardarin antibody
    • Leucine rich repeat kinase 2 antibody
    • leucine rich repeat serine threonine protein kinase 2 antibody
    • Leucine-rich repeat serine/threonine-protein kinase 2 antibody
    • LRRK 2 antibody
    • LRRK2 antibody
    • LRRK2_HUMAN antibody
    • PARK 8 antibody
    • PARK8 antibody
    • RIPK7 antibody
    • ROCO 2 antibody
    • ROCO2 antibody
    see all

图片

  • All lanes : Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) at 1 µg/ml

    Lane 1 : GFP-LRRK2 wt transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
    Lane 2 : GFP-LRRK2 mutant D2017A transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
    Lane 3 : GFP-LRRK2 mutant S1292A/G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose
    Lane 4 : GFP-LRRK2 mutant G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit (IRDye 800) at 1/10000 dilution

    Predicted band size: 286 kDa
    Observed band size: 313 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% BSA/TBST.

    The image is provided by Dr. Jeremy Nicols, Parkinson’s Institute, Sunnyvale, CA, USA.

    G2019S mutation results in an increased LRRK2 auto-phosphorylation including S1292 (lane 4).

    Observed band size: 286 + 27 (GFP) = 313 kDa.

  • All lanes : Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) at 1 µg/ml

    Lane 1 : LRRK2 mutant G2019S/S1292A transfected HEK293 lysate
    Lane 2 : LRRK2 mutant G2019S transfected HEK293 inhibitor treated lysate
    Lane 3 : LRRK2 mutant G2019S transfected HEK293 lysate
    Lane 4 : LRRK2 mutant S1292A transfected HEK293 lysate
    Lane 5 : LRRK2 wt transfected HEK293 inhibitor treated lysate
    Lane 6 : LRRK2 wt transfected HEK293 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2500 dilution

    Predicted band size: 286 kDa
    Observed band size: 286 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    The image is provided by Dr. Paul Davies, University of Dundee, Dundee Scotland, UK.

    G2019S mutation results in an increased LRRK2 auto-phosphorylation including S1292 (lane 3).

  • Characterization of pS1292 LRRK2 antibody

    Increasing amounts of HEK-293FT lysates transiently expressing WT LRRK2 (A) or S1292A LRRK2 (B) were analyzed by western blot and probed with the commercially available anti-pS1292 LRRK2 antibody (ab203181). The antibody showed minimal non-specific bands and linear detection in the range tested (b).

    From Figure1a of Kluss et al.

    Kluss et al  NPJ Parkinsons Dis. 2018; 4: 13. Published online 2018 Apr 19. doi: 10.1038/s41531-018-0049-1

    Reproduced under Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/.

实验方案

文献

This product has been referenced in:

  • Kim J  et al. LRRK2 kinase plays a critical role in manganese-induced inflammation and apoptosis in microglia. PLoS One 14:e0210248 (2019). Read more (PubMed: 30645642) »
  • Ho DH  et al. G2019s LRRK2 promotes mitochondrial fission and increases TNFa-mediated neuroinflammation responses. Anim Cells Syst (Seoul) 23:106-111 (2019). Read more (PubMed: 30949397) »
See all 13 Publications for this product

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