Anti-LRP1抗体(ab168454)
Key features and details
- Mouse polyclonal to LRP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-LRP1抗体
参阅全部 LRP1 一抗 -
描述
小鼠多克隆抗体to LRP1 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Recombinant fragment corresponding to amino acids 1-292 of Human LRP1. (AAH45107.1)
-
阳性对照
- Transfected 293T cell lysate; Human endometrium tissue; HeLa cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.4
Constituent: 100% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab168454于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa.
|
|
IHC-P |
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
Use a concentration of 10 µg/ml.
|
说明 |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa. |
IHC-P
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
-
功能
Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.
Functions as a receptor for Pseudomonas aeruginosa exotoxin A. -
组织特异性
Most abundant in liver, brain and lung. -
序列相似性
Belongs to the LDLR family.
Contains 22 EGF-like domains.
Contains 31 LDL-receptor class A domains.
Contains 34 LDL-receptor class B repeats. -
翻译后修饰
Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.
The N-terminus is blocked.
Phosphorylated on serine and threonine residues.
Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1. -
细胞定位
Cytoplasm. Nucleus. After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus and Cell membrane. Membrane, coated pit. - Information by UniProt
-
数据库链接
- Entrez Gene: 4035 Human
- Entrez Gene: 16971 Mouse
- Omim: 107770 Human
- SwissProt: Q07954 Human
- SwissProt: Q91ZX7 Mouse
- Unigene: 162757 Human
- Unigene: 271854 Mouse
-
别名
- A2MR antibody
- Alpha 2 macroglobulin receptor antibody
- alpha 2MR antibody
see all
图片
-
All lanes : Anti-LRP1 antibody (ab168454) at 1 µg/ml
Lane 1 : LRP1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-mouse HRP at 1/2500 dilution -
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling LRP1 with ab168454 at 10µg/ml.
-
Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human endometrium tissue labeling LRP1 with ab168454 at 3µg/ml.
实验方案
数据表及文件
-
Datasheet download
文献 (2)
ab168454 被引用在 2 文献中.
- Zheng Y et al. Anti-PAI-1 Monoclonal Antibody Inhibits the Metastasis and Growth of Esophageal Squamous Cell Carcinoma. J Cancer 14:114-128 (2023). PubMed: 36605486
- Ma J et al. Neutrophil elastase-regulated macrophage sheddome/secretome and phagocytic failure. Am J Physiol Lung Cell Mol Physiol 321:L555-L565 (2021). PubMed: 34261337