概述

  • 产品名称
    Anti-LRP1抗体[5A6]
    参阅全部 LRP1 一抗
  • 描述
    小鼠单克隆抗体[5A6] to LRP1
  • 宿主
    Mouse
  • 经测试应用
    适用于: Flow Cyt, WB, IHC-P, IHC-Fr, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human
  • 免疫原

    Recombinant fragment (Human), 85kDa cleaved fragment of Low Density LRP

  • 阳性对照
    • Ab28320 gave a positive signal in Western Blot in WI38 whole cell lysate. This antibody also gave a positive result in IHC in the following FFPE tissue: Human normal placenta.
  • 常规说明

    Western blot protocol advice:

    We do not recommend adding a reducing agent (e.g. DTT) to lysates or heating samples prior to loading on SDS-PAGE gels. We have used BSA as our blocking agent at 1%.

    This antibody clone is manufactured by Abcam. If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

应用

Our Abpromise guarantee covers the use of ab28320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

WB Use a concentration of 1 µg/ml. Use under non reducing condition. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).

Abcam do not recommend adding a reducing agent (e.g. DTT) to lysates or heating samples prior to loading on SDS-PAGE gels.  We have used BSA as our blocking agent at 1%.

IHC-P Use a concentration of 10 µg/ml.
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.
    Functions as a receptor for Pseudomonas aeruginosa exotoxin A.
  • 组织特异性
    Most abundant in liver, brain and lung.
  • 序列相似性
    Belongs to the LDLR family.
    Contains 22 EGF-like domains.
    Contains 31 LDL-receptor class A domains.
    Contains 34 LDL-receptor class B repeats.
  • 翻译后修饰
    Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.
    The N-terminus is blocked.
    Phosphorylated on serine and threonine residues.
    Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1.
  • 细胞定位
    Cytoplasm. Nucleus. After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus and Cell membrane. Membrane, coated pit.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A2MR antibody
    • Alpha 2 macroglobulin receptor antibody
    • alpha 2MR antibody
    • Alpha-2-macroglobulin receptor antibody
    • APOER antibody
    • Apolipoprotein E receptor antibody
    • APR antibody
    • CD 91 antibody
    • CD91 antibody
    • CD91 antigen antibody
    • IGFBP3R antibody
    • LDL receptor related protein 1 antibody
    • Low density lipoprotein receptor related protein 1 antibody
    • Low density lipoprotein related protein 1 antibody
    • Low-density lipoprotein receptor-related protein 1 intracellular domain antibody
    • LRP 1 antibody
    • LRP 515 antibody
    • LRP 85 antibody
    • LRP antibody
    • LRP ICD antibody
    • LRP-1 antibody
    • LRP-515 antibody
    • LRP-85 antibody
    • Lrp1 antibody
    • LRP1 protein antibody
    • LRP1_HUMAN antibody
    • LRP1A antibody
    • LRP515 antibody
    • LRP85 antibody
    • LRPICD antibody
    • MGC88725 antibody
    • Prolow density lipoprotein receptor related protein 1 antibody
    • TbetaR V/LRP 1/IGFBP 3 receptor antibody
    • TbetaRV/LRP1/IGFBP3 receptor antibody
    • TGFBR 5 antibody
    • TGFBR5 antibody
    • Type V tgf beta receptor antibody
    see all

图片

  • IHC image of LRP1 staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab28320, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-LRP1 antibody [5A6] (ab28320) at 1 µg/ml + WI38 (Human lung fibroblast cell line) Whole Cell Lysate [Non-reduced] at 25 µg

    Secondary
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 85 kDa
    Observed band size: 85 kDa


    Exposure time: 20 minutes


    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Bovine Serum Albumin before being incubated with ab28320 overnight at 4°C.  Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.  Please note that no reducing agent was added to the cell lysate and the sample was not heated prior to loading onto the SDS-PAGE gel. 

  • ab28320 at 1/3000 + WI38 fibroblasts whole cell lysate

    Secondary
    HRP conjugated donkey anti-mouse polyclonal diluted 1/10000

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 85 kDa
    Observed band size: 85 kDa


    Exposure time: 2 minutes


    50µg of human fibroblast whole cell lysate were loaded on to the gel and run under denaturing conditions.

    The membrane was blocked with 1% BSA for 1 hour at RT.

    The primary antibody was incubated for 12 hours at 4°C.

    See Abreview

  • ICC/IF image of LRP1 staining in rat mixed glia culture using ab28320 (1:500). The cells were fixed uisng paraformaldehyde and permeabilised using 0.3% TritonX in 0.1%PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. the Cells were then inubated with ab23020 for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to anti mouse IgG conjuagted to Alexa Fluor 568.

    See Abreview

  • Overlay histogram showing HepG2 cells stained with ab28320 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28320, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HepG2 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

文献

This product has been referenced in:
  • Willoughby JLS  et al. Evaluation of GalNAc-siRNA Conjugate Activity in Pre-clinical Animal Models with Reduced Asialoglycoprotein Receptor Expression. Mol Ther 26:105-114 (2018). WB . Read more (PubMed: 28988716) »
  • Chen J  et al. SLAMF7 is critical for phagocytosis of haematopoietic tumour cells via Mac-1 integrin. Nature 544:493-497 (2017). WB . Read more (PubMed: 28424516) »
See all 19 Publications for this product

客户评价及客户问答

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1-8 of 8 Abreviews

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Microglia)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
10 µg
Specification
Microglia
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. coralie brifault

Verified customer

提交于 Sep 13 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Mixed glial culture)
Specification
Mixed glial culture
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Dec 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

提交于 Nov 08 2011

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse lung)
Specification
mouse lung
Fixative
Methanol
Permeabilization
Yes - 0.1% Triton-X100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C

Abcam user community

Verified customer

提交于 Jun 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Vlady Pavlova

Verified customer

提交于 Dec 24 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Fibroblast)
Loading amount
50 µg
Specification
Fibroblast
Gel Running Conditions
Non-reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Dr. Torry Tucker

Verified customer

提交于 Nov 15 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (BeWo)
Loading amount
20 µg
Specification
BeWo
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

提交于 Mar 22 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HUVEC)
Specification
HUVEC
Fixative
Methanol
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%

Abcam user community

Verified customer

提交于 Mar 22 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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