Anti-LOX抗体(ab223488)
Key features and details
- Goat polyclonal to LOX
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-LOX抗体
参阅全部 LOX 一抗 -
描述
山羊多克隆抗体to LOX -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Pig -
免疫原
Synthetic peptide corresponding to Human LOX aa 200-300 (internal sequence) (Cysteine residue). NP_002308.2; NP_001171573.1.
Database link: P28300 -
阳性对照
- WB: Human placenta and heart lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab223488 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223488于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 30-33 kDa (predicted molecular weight: 46.9 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 30-33 kDa (predicted molecular weight: 46.9 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. -
组织特异性
Heart, placenta, skeletal muscle, kidney, lung and pancreas. -
疾病相关
Defects in LOX may be a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100]. -
序列相似性
Belongs to the lysyl oxidase family. -
翻译后修饰
The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine. -
细胞定位
Secreted > extracellular space. - Information by UniProt
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数据库链接
- Entrez Gene: 4015 Human
- Entrez Gene: 16948 Mouse
- Entrez Gene: 24914 Rat
- Omim: 153455 Human
- SwissProt: P28300 Human
- SwissProt: P28301 Mouse
- SwissProt: P16636 Rat
- Unigene: 102267 Human
see all -
别名
- lox antibody
- LYOX antibody
- LYOX_HUMAN antibody
see all
图片
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All lanes : Anti-LOX antibody (ab223488) at 0.5 µg/ml
Lane 1 : Human heart lysate in RIPA buffer
Lane 2 : Human placenta lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 46.9 kDa
Observed band size: 30-33 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
数据表及文件
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Datasheet download
文献 (1)
ab223488 被引用在 1 文献中.
- Chu Q et al. Extracellular matrix remodeling is associated with the survival of cardiomyocytes in the subendocardial region of the ischemic myocardium. Exp Biol Med (Maywood) 246:2579-2588 (2021). PubMed: 34515546