Anti-Livin抗体(ab97350)
Key features and details
- Rabbit polyclonal to Livin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Livin抗体
参阅全部 Livin 一抗 -
描述
兔多克隆抗体to Livin -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-211 of Human Livin (AAH14475).
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阳性对照
- WB: A431, H1299, HeLa and HepG2 whole cell lysate (ab7900) IHC-P: Cal27 xenograft ICc/IF: HeLa cell
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97350于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 33 kDa.
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| IHC-P |
1/100 - 1/500.
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| ICC/IF |
1/100 - 1/200.
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| 说明 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 33 kDa. |
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IHC-P
1/100 - 1/500. |
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ICC/IF
1/100 - 1/200. |
靶标
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功能
Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits caspase-3 and proteolytic activation of pro-caspase-9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis. -
组织特异性
Isoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes. -
序列相似性
Belongs to the IAP family.
Contains 1 BIR repeat.
Contains 1 RING-type zinc finger. -
细胞定位
Nucleus. Cytoplasm. Nuclear, and in a filamentous pattern throughout the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 79444 Human
- Omim: 605737 Human
- SwissProt: Q96CA5 Human
- Unigene: 256126 Human
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别名
- Baculoviral IAP repeat containing 7 antibody
- Baculoviral IAP repeat containing protein 7 antibody
- Baculoviral IAP repeat-containing protein 7 antibody
see all
图片
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Western blot - Anti-Livin antibody (ab97350)Anti-Livin antibody (ab97350) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 33 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Livin antibody (ab97350)ab97350, at a 1/200 dilution, staining Livin in paraformaldehyde fixed HeLa cell by immunofluorescence.
The lower image shows ab97350 merged with DNA probe. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Livin antibody (ab97350)ab97350, at a 1/100 dilution, staining Livin in paraffin embedded Cal27 xenograft by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab97350 被引用在 9 文献中.
- Chen T et al. MUC 15 Promotes Osteosarcoma Cell Proliferation, Migration and Invasion through Livin, MMP-2/MMP-9 and Wnt/ß-Catenin Signal Pathway. J Cancer 12:467-473 (2021). PubMed: 33391443
- Jin CY et al. High expression of RRM2 as an independent predictive factor of poor prognosis in patients with lung adenocarcinoma. Aging (Albany NY) 13:3518-3535 (2020). PubMed: 33411689
- Duan WJ et al. MiR-512-3p regulates malignant tumor behavior and multi-drug resistance in breast cancer cells via targeting Livin. Neoplasma 67:102-110 (2020). PubMed: 31777256
- Gao LM et al. Tumor-suppressive effects of microRNA-181d-5p on non-small-cell lung cancer through the CDKN3-mediated Akt signaling pathway in vivo and in vitro. Am J Physiol Lung Cell Mol Physiol N/A:N/A (2019). PubMed: 30628487
- Gao XL et al. Effects of RNA interference-mediated silencing of toll-like receptor 4 gene on proliferation and apoptosis of human breast cancer MCF-7 and MDA-MB-231 cells: An in vitro study. J Cell Physiol 234:433-442 (2018). PubMed: 29932226
- Liu S et al. Silencing Livin improved the sensitivity of colon cancer cells to 5-fluorouracil by regulating crosstalk between apoptosis and autophagy. Oncol Lett 15:7707-7715 (2018). PubMed: 29740490
- Su QB et al. Livin serves as a prognostic marker for mid-distal rectal cancer and a target of mid-distal rectal cancer treatment. Oncol Lett 14:7759-7766 (2017). PubMed: 29344221
- Saladi SV et al. BRG1 promotes survival of UV-irradiated melanoma cells by cooperating with MITF to activate the melanoma inhibitor of apoptosis gene. Pigment Cell Melanoma Res 26:377-91 (2013). WB . PubMed: 23480510
- Li X et al. RNA interference-mediated knockdown of Livin suppresses cell proliferation and invasion and enhances the chemosensitivity to cisplatin in human osteosarcoma cells. Int J Oncol 43:159-68 (2013). PubMed: 23632777